Analysis of commensal neisserial species as a genetic pool for drug resistant genes

各种抗微生物病原体已经出现。除了引起医院感染的病原体外，社区获得性感染的病原体也产生了抗菌素耐药性。在这个项目中，我们试图了解抗性基因的出现和获得模式。我们分析了共107株淋病奈瑟菌，推测它们是淋病奈瑟菌耐药基因的来源。在商业菌株中检测到头孢曲松（CRO）耐药基因。从penA基因比较来看，新鉴定的耐cro共生菌株的penA与penA （H041）相似（不完全相同）。在penA （H041）中观察到的一些独特氨基酸与抗CRO菌株共享。设计了一套能特异检测penA （H041）的实时PCR检测系统。我们尝试在CRO治疗淋病奈瑟菌后检测出耐CRO的奈瑟菌系菌株，但未出现耐药菌株。

Various kinds of antimicrobial-resistant pathogens have emerged. In addition to pathogens causing nosocomial infections, pathogens for community-acquired infections also have developed their antimicrobial-resistance.n this project, we tried to understand the mode of emergence and acquisition of resistant gene. We analyzed total 107 commesal neisserial strains, which are speculated as a source of resistant genes for N. gonorrhoeae. We could detect ceftriaxone (CRO) resistant genes in the commesal strains. From the comparison of penA genes, newly identified penA from CRO-resistant commensal strains were similar (not identical) with penA (H041). Some unique amino acids observed in penA (H041) were shared with the CRO resistant strains. We designed real-time PCR assay system, which could detect penA (H041) specifically. We tried to detect CRO-resistant neisserial strains after CRO treatment for N. gonorrhoeae, but the resistant strains could not be emerged.