Technical development of microscopic replantation of animal leg frozen with CAS freezer.

冷冻过程导致重要组织中的细胞完全死亡。细胞存活系统（CAS）是一种特殊的冷冻技术，其在冷冻期间将细胞死亡抑制在最低限度。本研究的目的是发展断肢、CAS冻融动物腿再植技术。结果，虽然再植的腿最终导致完全坏死，但为将来提供了非常有用的发现。也就是说，CAS冷冻机性能的进步，以产生更快和更均匀的冷冻，以及冷冻保存溶液的调整和制备，将导致未来的成功。

Usual freezing process causes completely cell death in the vital tissue. Cells Alive System (CAS) is a particular freezing technique which restrains cell death during freezing at minimum. Purpose of this research was to develop replantation technique of amputated, CAS frozen, and thawed animal leg. As results, although replanted leg resulted in complete necrosis eventually, very useful findings for the future were achieved. That is, progress of performance of CAS freezer as to produce quicker and more uniform freezing, and adjustment and preparation of cryopreservation solution would lead to future success.