Periodontal ligament cell growth with their stemness on reconstituted extracellular matrices mimicking stem cell niche

采用脱细胞技术制备由牙周韧带细胞（pdl）、间充质干细胞（MSCs）和成纤维细胞（NHDFs）形成的细胞外基质作为细胞培养底物。肌动蛋白纤维和细胞核在脱细胞后不能被观察到，而在脱细胞前可以清晰地观察到，这表明细胞成分从底物中被去除。pdl可以附着在这些脱细胞矩阵上。pdl衍生的脱细胞基质比MSCs和nhdfs衍生的脱细胞基质附着更多的pdl。PDL也可以在这些脱细胞基质上生长。然而，pdl在pdl和mscs衍生的脱细胞基质上的生长速率低于nhdf衍生的脱细胞基质。这些脱细胞基质有望在体内作为模拟PDL周围生态位的基质。

Extracellular matrices formed by periodontal ligament cells (PDLs), mesenchymal stem cells(MSCs) and fibroblasts (NHDFs) were prepared as cell culture substrates by decellularization technique. Actin fibers and cell nuclei could not be observed after decellularization whereas they were clearly observed before decellularization, indicating that cellular components were removed from the substrates.PDLs can attach on these decellularized matrices. More PDLs attached on PDL-derived decellularized matrices than MSCs- and NHDFs-derived decellularized matrices. PDL can also grow on these decellularized matrices. However, the growth rates of PDLs on PDLs- and MSCs-derived decellularized matrices were lower than that on NHDF-derived decellularized matrices. It is expected that these decellularized matrices can be used as the substarates mimicking niche surrounding PDL in vivo.