Chemo-enzymatic Manipulation of RNA: Sequence-specifity via Cooperative Effects (B18*)

对mRNA的序列特异性操作提供了在转录后水平上研究和控制基因表达的途径，这对于分子生物学和细胞应用特别重要。5‘帽存在于所有真核生物的mRNAs中，而m6A是mRNAs中含量最丰富的内部修饰。这两种修改都控制着许多相互作用，但目前不能以特定于序列的方式安装或移除。该项目旨在通过创建RNA修饰酶与RNA结合蛋白或RNA-蛋白质复合体的融合构建物，在这些修饰中获得序列特异性的mRNA操纵。修饰的精确操作将被用来标记或分离特定的转录本，并评估生物学效应，特别是翻译效率和稳定性。

Sequence-specific manipulation of mRNA provides access to studying and controlling gene expression at the post-transcriptional level which is of particular importance for molecular biology and cellular applications. The 5′ cap is present in all eukaryotic mRNAs and m6A is the most abundant internal modification in mRNA. Both modifications control numerous interactions but currently cannot be installed or removed in a sequence-specific manner. This project aims to obtain sequence-specific mRNA manipulation at these modifications by creating fusion constructs of RNA-modifying enzymes with RNA-binding proteins or RNA-protein complexes. The precise manipulation of modifications will be used to label or isolate a specific transcript and to evaluate biological effects, particularly translation efficiency and stability.