ATP amplification reaction in pico-liter chamber and its application to bacteria detection.

为了快速测量溶液中微生物的数量，我们尝试使用毛细管阵列，这是一种圆形玻璃板，其细孔在二维阵列中有序排列。在含有atp提取试剂和生物发光试剂的毛细管阵列中加入一定量的充分稀释溶液后，可以很容易地从发光孔的比例中估计出微生物的数量。在本研究中，我们将荧光素酶固定在微球（直径约2.8 μm）上，微球可以作为溶液中微生物的模型。使用微珠，我们确定ATP的浓度需要产生可检测的发光水平。我们得出结论，微生物的快速测量需要进一步改进ATP扩增技术和使用高发光荧光素酶。

To rapidly measure the number of microorganisms in solution, we attempted to use a capillary array, a circular glass plate with fine pore regularly arranged in two-dimensional array. After applying a given amount of sufficiently diluted solution to the capillary array containing both ATP-extracting and bioluminescence reagents, the number of microorganisms can be easily estimated from the proportion of luminescent pores. In this research, we immobilized luciferase on micro-beads (approximately 2.8 μm in diameter) that could serve as a model of microorganisms in solution. Using the micro-beads, we determined the concentration of ATP needed to generate detectable level of luminescence. We concluded that the rapid measurement of microorganisms would require further improvement of ATP amplification technology and the use of high luminescence luciferase.