Investigation of the mechanisms of blue light-induced biological effects on skin and skin cells

最近的研究表明，除了紫外线辐射外，可见光还具有生物效应。我们首次证明了蓝光(410、420、453 nm)以剂量和波长依赖的方式影响人真皮成纤维细胞的增殖。此外，较短波长(410、420 nm)的照射可剂量依赖性地诱导细胞毒性，而非毒性剂量和波长为453 nm的照射可抑制细胞增殖并降低抗氧化能力。此外，我们还发现蓝光照射抑制了转化生长因子-β1诱导的成纤维细胞向肌成纤维细胞的分化。作为一种可能的机制，蓝光诱导产生的活性氧物种被假定，然而，无论是分子机制还是参与的信号通路都没有充分阐明。在这个项目的范围内，我们想要进一步表征蓝光对真皮成纤维细胞、皮肤标本和分化的影响，并确定涉及的机制和途径。此外，蓝光治疗的效果将在人体皮肤标本的3D伤口愈合模型中进行研究。对于一种可能的临床方法，将评估安全性方面，如蓝光导致DNA损伤或角质形成细胞功能降低，以及再上皮化的延迟。我们期待这一项目的有趣结果，这反过来可能与治疗和预防Dupuytren痉挛、肥厚性瘢痕、瘢痕疙瘩和硬皮病以及皮肤光老化有关。

Recent studies have shown that apart from UV radiation also visible light exerts biological effects. For the first time, we could demonstrate that blue light (410, 420, 453 nm) affects proliferation of human dermal fibroblasts in a dose- and wavelength-dependent manner. In addition, irradiation with shorter wavelengths (410, 420 nm) dose-dependently induces cell toxicity, whereas non-toxic doses and wavelength of 453 nm are able to inhibit proliferation and reduce the antioxidative capacity. Moreover, we found an inhibition of TGF-beta1-induced differentiation of fibroblasts into myofibroblasts by blue light irradiation. As a possible mechanism for the observed effects, the blue light-induced generation of reactive oxygen species are assumed, however, neither the molecular mechanisms nor the participating signal pathways are sufficiently elucidated. Within the scope of this project, we want to further characterize blue light-induced effects on dermal fibroblasts, skin specimen and differentiation, and identify the involved mechanisms and pathways. Moreover, the efficacy of blue light treatment will be investigated in a 3D wound healing model an human skin specimen. For a possible clinical approach, safety aspects such as blue light-induced DNA damages or reduced function of keratinocytes coupled with a delay of re-epithelisation will be assessed. We expect interesting results from this project, which in turn may relevant for the treatment and prevention of Dupuytren contractures, hypertrophic scars, keloids and scleroderma as well as skin photoaging.