Pathogenomics and eco-epidemiology of Mycobacterium orygis, an emerging zoonotic tuberculosis organism

为了研究人畜共患结核病（TB）新病原菌稻分枝杆菌（Mycobacterium orygis）的致病组学，我们对稻分枝杆菌（Mycobacterium orygis）进行了比较全基因组分析。我们获得的orygis分离物。我们的研究结果表明，M. Orygis已经形成了不同于其他M.肺结核综合征例如，与M.牛支原体的抗Sigk基因存在不同的突变模式。Orygis，导致产生更高的mpt 70和mpt 83免疫原性蛋白。gyrB、mmpL6、PPE 55、Rv2042c、TbD1基因多态性为M。Orygis specific.这些遗传特征为发展特异性检测支原体的快速诊断方法提供了可能。奥瑞吉斯我们正在继续对我们的M进行深入的全基因组分析。通过仔细筛选每一个单核苷酸多态性、插入和缺失以及遗传重组来确定它们与M.我们已经开发了一种粪便分枝杆菌测定法，其使用磁珠捕获TB DNA，其随后可以通过LAMP方法检测。当我们在尼泊尔进行实地验证时，我们在两头结核血清阳性的大象身上检测到了结核菌。我们已经分离出四个M。从四头圈养大象死后疑似结核病肺组织样本的培养物中分离出结核菌。我们合作监测尼泊尔的人畜共患结核病。但是，我们无法检测到M。在尼泊尔从疑似人类结核病样本中分离出的大猩猩和其他人畜共患结核菌。

To investigate the pathogenomics of Mycobacterium orygis, an emerging zoonotic tuberculosis (TB) pathogen, we conducted a comparative whole genome analysis of M. orygis isolates that we had obtained. Our findings suggest that M. orygis has developed unique pathogenic strategies that are differ from other members of M. tuberculosis complex. For instance, unlike in M. bovis, there is a different mutation pattern in the anti-Sigk of M. orygis, leading to higher production of mpt70 and mpt83 immunogenic proteins. Furthermore, polymorphisms in gyrB, mmpL6, PPE55, Rv2042c, TbD1 were identified to be M. orygis specific. These genetic characteristics offer the potential for the development of rapid diagnostic methods for the specific detection of M. orygis. We are continuing our in-depth whole genome analysis of our M. orygis isolates by carefully scruntinizing each single nucleotide polymorphisms, insertion and deletion, and genetic recombination to determine their association with M. orygis.We have developed a fecal mycobacterial assay that uses magnetic beads to capture TB DNA, which can later be detected by the LAMP method. When we validated in a field setting in Nepal, we detected TB bacteria in two TB-seropositive elephants. We have isolated four M. tuberculosis isolates from the culture of postmortem TB-suspected lung tissue samples of four captive elephants. We have collaborated for the surveillance of zoonotic TB in Nepal. However, we were unable to detect M. orygis and other zoonotic TB bacteria from human tuberculosis suspected samples in Nepal.