Development of technology to control osteogenic differentiation of iPS cells using shear-stress loading

1. 与静态组相比，剪切应力（0.05、0.5和1.5 Pa）增强了腓骨干细胞的成骨分化2。0.5 Pa诱导多能干细胞中胚层和内胚层分化，抑制外胚层分化。在中胚层衍生物中，内皮细胞标记物在剪切胁迫下显著上调。0.5 Pa抑制OPG基因表达，但有趣的是促进了成骨诱导的ipsc对OPG的释放。相比之下，0.5 Pa也增强了RANKL基因和蛋白的表达。

1. Shear stress (0.05,0.5, and 1.5 Pa) enhanced osteogenic differentiation ofiPSCs compared to the static group2. 0.5 Pa induced mesoderm and endoderm differentiation of iPSCs while suppressingectoderm differentiation. Among mesodermal derivatives, endothelial cell markers were markedly upregulated by shear stress.3. 0.5 Pa suppressed OPG gene expression but interestingly enhanced the release of OPG by osteogenically-induced iPSCs. In contrast, 0.5 Pa also enhanced RANKL gene and protein expressions.