Development of novel Immunosynbiotics with wakame utilizing immunobiotics and immunogenics

在畜牧业生产中过度使用抗生素造成的抗生素耐药性一直是一个严重的问题，导致需要有效的替代品。抗生素的一种有希望的替代品是“免疫合成生物制剂”，它是免疫生物制剂和免疫益生菌的结合。海藻WAKAME被认为是一种免疫增生剂，而唾液乳杆菌是一种免疫生物，从WAKAME喂养的猪的肠道中分离出来。已发现在猪饲料中添加WAKAME可以改善免疫反应，并改变猪大肠中的微生物区系。使用WAKAME作为饲料补充剂也可以为2011年东北地震后宫城县的经济复苏提供另一种选择。今年，这项研究取得了三个结果：(1)在以前的研究中选择的8株乳酸菌能够在添加B族维生素的WAKAME发酵液中生长得更好。比较基因组学分析表明，菌株间的差异与其利用wakame的能力不同有关。(2)由于wakame中的纤维可以保护细胞免受严酷的酸环境的伤害，所以能够在含有wakame的模拟胃液中存活。(3)生长和储存条件对乳酸菌的特性有不同的影响，尤其是高温和低温胁迫。在未来的研究中，确定的培养条件下的菌株将为体内研究提供依据。

Antibiotic resistance caused by the overuse of antibiotics in livestock production has been a severe problem, leading to the need for effective alternatives. A promising alternative to antibiotics is the "immunosynbiotics," a combination of immunobiotics and immunoprebiotics. The seaweed wakame is considered an immunoprebiotic, and L. salivarius is an immunobiotic, isolated from the intestine of wakame-fed pigs. Adding wakame to pig feed has been found to improve the immune response and alter the microbiota in the porcine large intestine. Using wakame as a feed supplement could also provide an economic recovery alternative in Miyagi prefecture after the 2011 Tohoku earthquake. This year, three results were achieved in this study:(1)Eight lactobacilli strains selected in previous studies were able to grow better in wakame-based broths with the addition of B vitamins. The comparative genomic analysis revealed that the differences between the strains were related to their different wakame utilizing abilities.(2)One strain that had adhesion ability to porcine intestinal epithelial cells could survive under the simulated gastric juice in the presence of wakame because the fiber in wakame could protect the cells from the severe environment of acid.(3)The growth and storage conditions have various effects on the characteristics of lactic acid bacteria, especially high-temperature and low-temperature stress The incubation condition of the selected strain was determined. In future research, the strain under determined incubation conditions will be provided for the in vivo study.