Hybridization kinetics and non-equilibrium dynamics of random pools of oligonucleotides (P15)

我们将从实验和理论上研究寡核苷酸池中的杂交动力学，并研究池的组成-单链，部分杂交，非同源复合物和“完美匹配”复合物-如何随时间变化。由于杂交在很大程度上依赖于盐浓度和pH等环境参数，因此我们将研究盐梯度内序列池的时空动态。我们将开发实验方法来监测不同地点池内的不同物种，并开发理论工具来分析这些复杂的动态。

We will study, both experimentally and theoretically, hybridization kinetics within oligonucleotide pools and investigate how the composition of the pool – single-strands, partially hybridized, non-cognate complexes, and “perfectly matched” complexes – changes over time. As hybridization is strongly dependent on environmental parameters such as salt concentration and pH, we will then study the spatiotemporal dynamics of the sequence pools within salt gradients. We will develop experimental methods to monitor different species within the pool at different locations, and theoretical tools to analyze these complex dynamics.