Molecular analysis of novel wound-induced regulators of shoot regeneration in Arabidopsis

在2022财年，我对该项目的主要贡献是表征伤口激活的热休克因子A1（HSFA 1）如何促进拟南芥的芽再生。本研究综合运用遗传学、生物化学和生物信息学等多种手段，揭示了创伤诱导再生的新机制。主要研究成果：1）利用生物信息学方法确定了HSFA 1的下游关键靶基因。2)使用生物化学验证HSFA 1与直接靶标的结合。3)产生和表型遗传杂交，以确认参与创伤诱导细胞重编程的基因上位性。4)观察到HSFA 1d的核易位，以响应使用活的创伤-结果已汇编成手稿提交2023年合作（非正式）1）阿里Sadanandom（达勒姆大学，英国）共享植物材料，并共同撰写了一份拨款建议，生物技术和生物科学研究理事会（BBSRC），以调查细胞重编程中的SUMO化。2)Teruaki Taji（Tokyo University of Agriculture）提供cDNA样品用于qPCR分析，以研究HSFA 1在光形态建成中的作用。3)儿玉丰（宇都宫大学）参观了实验室，学习了用共聚焦显微镜对叶表皮细胞进行成像的技术。会议：1）Oral -法国蒙彼利埃实验生物学学会年会。2）Oral -法国蒙彼利埃实验生物学学会SUMO修饰细胞信号的卫星会议特邀演讲人。3)海报- CSRS 2022年度进展会议

In FY 2022 My main contribution to this project is characterizing how the wound-activated HEAT SHOCK FACTOR A1 (HSFA1) promotes shoot regeneration in Arabidopsis. I utilized a combination of genetic, biochemical and bioinformatic tools to reveal a novel mechanism of wounding-induced shoot regeneration.Key research findings: 1) Determined key downstream targets of HSFA1 using a bioinformatics approach. 2) Verified HSFA1 binding to direct targets using biochemistry. 3) Generated and phenotyped genetic crosses to confirm epistasis of genes involved in wound-induced cellular reprograming. 4) Observed the nuclear translocation of HSFA1d in response to wounding using live -The results have been compiled into a manuscript for submission in 2023Collaborations (informal)1) Ari Sadanandom (Durham University, UK) – Shared plant material and jointly wrote a grant proposal to Biotechnology and Biological Sciences Research Council (BBSRC) to investigate SUMOylation in cellular reprograming. 2) Teruaki Taji (Tokyo University of Agriculture) – Provided cDNA sample for qPCR analysis to investigate the role of HSFA1 in photomorphogenesis. 3) Yutaka Kodama (Utsunomiya University) – Visited laboratory and learned techniques for imaging leave epidermal cells with a confocal microscope.Conferences: 1)Oral - Annual conference for Society of Experimental Biology, Montpellier France.2) Oral - Satellite meeting on SUMO modification in cell signalling – Society of Experimental Biology, Montpellier France – Invited speaker. 3)Poster - CSRS Annual progress meeting 2022