Point-of-care therapeutic monitoring of anti-bacterial using low-cost disposable sensor

监测抗微生物药物的水平对于避免毒性效应至关重要。治疗药物监测(TDM)是实现这一目标的有用工具。我们开发了一种万古霉素(VCM)传感器来监测全血中的药物浓度。然而，电流传感器的稳定性和VCM的灵敏度取决于芯片的基座结构和安培测量方式。以前，我们有两个主要问题：分子印迹聚合物接枝碳糊(MIP-CP)不可重复性，以及芯片传感器在包装后三天内失去灵敏度。经过多次优化，我们成功地制备了重复性很好的MIP-CP。该芯片具有良好的批次重复性和长期稳定性。此外，包装好的芯片在缓冲盐水中的长期稳定性提高到两周，在全牛血中的稳定性提高到十天。这标志着比过去的芯片传感器有了很好的改进。通过三次会议成功地传播了这些数据。

Monitoring the levels of antimicrobial drugs is crucial to avoid toxic effects. Therapeutic drug monitoring (TDM) is a helpful tool to achieve this. We have developed a vancomycin (VCM) sensor to monitor drug concentration in whole blood. However, current sensor stability and VCM sensitivity depend on chip base structure and amperometric measurement mode. Previously, we had two major problems: the molecularly imprinted polymer-grafted-carbon paste (MIP-CP) was not reproducible, and the chip sensor lost sensitivity within three days of packing. After several optimizations, we have successfully made a highly reproducible MIP-CP. This chip has good batch reproducibility and good long-term stability. Also, the long-term stability of the packed chip has improved to two weeks in buffer saline and ten days in whole bovine blood. This indicates a good improvement over the past chip sensor. The data has been successfully disseminated through three conferences.