Development of functional evaluation for cartilagenous matrix using the measurement of ^1H-NMR and viscoelasticity

为了测量软骨组织的1H-NMr弛豫时间和粘弹性模量，在强磁场的核磁共振装置（型号：JNM 60E FSE， JEOL, Tokyo, Japan）中布置并设置粘弹性光谱仪。分析了牛膝关节股骨髁全深度软骨和牛椎间盘髓核中h - nmr弛豫时间与粘弹性模量的关系。粘弹性能谱仪的测量探头设计适应核磁共振磁体的狭窄空间，使机械换能器系统由水平地层变为垂直地层。动态力发生器由在线计算机系统（型号：PC9801VM2， NEC, Tokyo, Japan）控制，并对通过软骨接收到的测力信号进行分析，计算粘弹性模量。对于关节软骨，水的h - nmr弛豫次数主要随基质中含水量的变化而变化。应力疲劳试验过程中，随着基体中水分向基体外空间扩展，1H-NMR松弛次数增加，粘弹性模量也增加到平台值。由于取样区域内相对增加的自由水比其基体中的束缚水具有更长的1H-NMR弛豫时间，因此在此过程中最终观察到的弛豫时间增加。髓核的粘弹性取决于水的流动性，这种流动性由1H-NMR弛豫时间表示。制备了含两种不同量糖胺聚糖的牛椎间盘髓核。正常组的结果表明，h - nmr弛豫时间与粘弹性模量之间存在线性关系，而酶解退化组的结果表明，h - nmr弛豫时间与粘弹性模量之间不存在线性关系，粘弹性模量似乎不仅取决于水的流动性，还取决于胶原纤维的弹性。综上所述，糖胺聚糖通过保留水分和限制其流动性，在软骨的粘弹性性能中起重要作用。结果表明，通过测定软骨基质中水的迁移率，可以估计软骨组织的基质紊乱和软骨组织的功能评价。少

For the measurement of 1H-NMr relaxation times and visocelastic modules of cartilagenous tissue, viscoelastic spectrometer was arrenged and set up in the strong magnetic field of NMR devices( Model: JNM 60E FSE, JEOL, Tokyo, Japan). Relationship of 1H-NMR relaxation times and viscoelastic modules were analized in the full depth cartilage prepared from the femoral condyles of the knee joints of the cow, and in the nucleus pulposus of the intervertebral discs of the cow. The measuring probe of the viscoelastic spectrometer was designed to fit the narrow space in the NMR magnet, so that the mechanical transducer systems was changed into vertical formation from horizontal formation. Dynamic force generators were controlled by on-line computer system( Model: PC9801VM2, NEC, Tokyo, Japan) and the recieved signals of the detected fofce through the cartilage were analized and the viscoelastic modules were calculated by the computer.As for the joint cartilage, 1H-NMR relaxation times of water a … More nd viscoelastic modules were changed mainly with the change in the water contents in the matrix. As the water in the matrix exdated to the extra-matrix space during the stress fatigue test, 1H-NMR relaxation times increased and viscoelastic modules also increased to the plateau values. Because the free water fraction relativelly increased in the sampling area had longer 1H-NMR relaxation times than the bound water in its matrix, the observed relaxation times increased in this process finally. Viscoelastic property in the nucleus pulposus depended on the mobilities of water, which were represented by 1H-NMR relaxation times. Nucleus pulposus of the cow disos containing two different amount of glycosaminoglycans were prepared. The results of normal group indicated the liner relationship between 1H-NMR relaxation times and viscoelastic modules, while in the enzimatically degenerated group the liner relationship was lost and viscoelastic modules seemed to depended not only on the mobilities of water but also on the elasticity of collagen fiber.In conclusion, glycosaminoglycans plays an important part in the viscoelastic property of the cartilage by retaining water and resrticting its mobilities. It was suggested that the estimation of matrix derangement and functional evaluation of cartilagenous tissues could be possible by measuring of the mobilities of water in its matrix. Less