Basic and clinical research for periodontal disease of physiologically vasoactive substances extracted from skeletal muscle of fur seal

我们先前已经检测了从海狗骨骼肌水解物中提取的肽样生理活性物质的外周血管舒张作用，并且发现肽样物质显示犬股动脉和腹部皮肤的血流量增加（Matsukawa等人，公牛。日本。社会科学鱼. 40：1139，1974）。此外，肽样物质产生犬牙龈血流量的增加（Tsujitani，神奈川志高18：125，1983），这表明假定该性质对发炎的牙周组织有益。当制备含有肽样物质的糊剂并在临床实践中应用于牙周病以测试这一点时，观察到牙龈状况的显著改善。随着时间的推移，注意到发红、肿胀、引流、出血和囊袋深度的明显临床改善（Hiyama等人，J. Periodontol. 53：639，1982）。然而，PEPT的精确结构和/或组分 ...更多信息 从海狗骨骼肌中提取的类IDE物质尚不清楚，其结构与生理活性之间的关系尚需进一步研究。为了进一步了解该物质的药理作用，本研究建立了从海狗骨骼肌中提取该物质的方法，并对以下提取步骤进行了试验。首先确定了海狗肌肉中活性物质的提取效率，其次确定了粗提物中活性物质的分级分离方法和分级产物的收集方法。血管活性物质大量存在于分子量小于1,000 M.W.的部分，但不能超过1,000兆瓦。对于分级方法，用Amberlite XAD-2树脂吸附层析法从海狗骨骼肌粗提物中分离出高活性组分，而不损失血管活性，将海狗骨骼肌粗提物经Amberlite XAD-2树脂层析分离得到的组分1进一步分为4个组分（A ~ D）。符合电密度离子交换色谱法。对每个级分进行表征，并获得以下结果。组分A、B、C或D分别被鉴定为5 '-ADP、5'-AMP、肌肽和鹅肌肽或组胺。据推测，从海狗骨骼肌中提取的肽样物质（粗混合物）对外周循环障碍的改善是由于所鉴定的血管活性物质，特别是腺苷衍生物之间的相互作用。少

We have previously examined peripheral vasodilating action of peptide-like physioactive substance extracted from the hydrolysate of skeletal muscle of fur seal, and found that the peptide-like substance shows an increase in blood flow of canine femoral artery and of abdominal skin (Matsukawa et al., Bull. Jpn. Soc. Sci. Fish. 40: 1139,1974). Furthermore, the peptide-like substance produces an increase in canine gingival blood flow (Tsujitani, Kanagawa Shigaku 18: 125, 1983), suggesting that this property is postulated to be beneficial to inflamed periodontal tissue. When a dentifrice containing the peptide-like substance was prepared and applied to periodontal disease in clinical practice to test this, significant improvement in the gingival conditions was observed. As time progressed, distinct clinical improvement in redness, swelling, drainage, bleeding and pocket depth was noted (Hiyama et al., J. Periodontol. 53: 639, 1982). However, precise structure and/or constituent of the pept … More ide-like substance extrated from skeletal muscle of fur seal are unknown, and relation between the structure and physiological activity requires clarification. To gain further insight into the pharmacological effect of the substance, the present study focuses on establishment of the method for extraction of the substance from skeletal muscle of fur seal.The following extractive steps were tested. The first step was to determine the procedure for the extractive efficiency of the substance from the muscles of fur seals; and the second was to detect the method of fractionating the active substance and of collecting the fractionate substance from crude extract. The vasoactive substances exsisted abundantly in the fraction of the molecular range less than 1,000 M.W., but not in that more than 1,000M.W. As for fractionating method, utilization of Amberlite XAD-2 resin absorptive chromatography was the best way to separate the high active fraction without a loss of vasoactive activity from crude extract mixture containing high molecular- and low active-fraction.The fraction 1 fractionated by amberlite XAD-2 resin chromatography from crude mixture extracted from skeletal muscle of fur seals was further divided broadly into 4 fractions (A to D) in compliance with electrical density ion exchange chromatography. Each fraction was characterized and following results were obtained. Fraction A, B, C, or D was identified as 5'-ADP,5'-AMP, carnosin and anserine, or histamine, respectively. it is postulated that improvement of peripheral circulatory disorders by the peptide-like substance (crude mixture) extracted from skletal muscle of fur seals is due to the interaction among the vasoactive substances, particularly adenosine derivatives, identified. Less