Impact of exclusive enteral nutrition on microbiome signatures and function in pediatric Crohn’s disease (P01)

克罗恩病(CD)是特发性炎症性肠病(IBD)的两大主要病因之一。在第二个资助阶段，该串联项目将具体说明饮食引起的肠道微生物群的变化，以实现完全肠内营养(EEN)对儿童克罗恩病的治疗效果。将使用连续培养、诺生菌小鼠和猪模型相结合的方法来表征与EIN相关的特征。在此背景下，将评估膳食纤维在引发疾病复发方面的作用。将对治疗反应和难治性患者进行纵向细菌和代谢物分析，以确定对EEN做出反应的功能相关微生物组目标，从而开发保护性合成细菌联合体。本项目的目标是为儿童CD患者开发一种基于微生物组的维持疗法。

Crohn’s disease (CD) is one of the two main entities of idiopathic inflammatory bowel disease (IBD). In the second funding phase, the tandem will specify the diet-induced changes in the intestinal microbiome for therapeutic efficacy of exclusive enteral nutrition (EEN) in pediatric Crohn’s disease. EEN-associated signatures will be characterized using the combined approach of continuous culture, gnotobiotic mice and pig models. In this context, the role of dietary fiber in provoking disease relapse will be evaluated. Longitudinal bacterial- and metabolite-profiling of treatment responsive and refractory patients will be implemented to identify functionally relevant microbiome targets in response to EEN, leading to the development of protective synthetic bacterial consortia. The perspective of this project is to develop a microbiome-based maintenance therapy for pediatric CD patients.