Selective plane illumination microscope (SPIM) with IT-equipment

我们部署在体内共聚焦成像技术在两个主要领域：（i）在植物中的运输过程，和（ii）小分子和生物化学/生物物理过程的生物传感器。我们系统地鉴定了负责离子和代谢物的吸收和释放的蛋白质，并描述了它们在植物生理学中的作用。为了表征转运蛋白的亚细胞定位和调节，我们使用共聚焦成像技术。我们还开发和部署遗传编码的荧光生物传感器，用于体内离子，代谢物和信号分子。我们率先开发了运输活动传感器。为了能够记录传感器输出，我们需要高灵敏度和定量的荧光成像技术。在我之前在斯坦福大学的实验室里，我们使用旋转圆盘和选择性平面照明显微镜来研究转运蛋白调节和生物传感器响应。该申请要求提供资金，用于一套两台荧光显微镜以及一个图像数据存储和处理系统。经过对多种仪器的仔细测试和评估，我们希望获得：[...]这些系统不仅将由我们的团队使用，还将集成到杜塞尔多夫的CAi（高级成像中心）中，从而使广大科学家都能使用。此外，我们还包括在大学和诊所的5个额外的团队的需求的具体说明。

We deploy in vivo confocal imaging techniques in two major areas: (i) Transport processes in plants, and (ii) biosensors for small molecules and biochemical/biophysical processes. We systematically identify proteins that are responsible for the uptake and release of ions and metabolites and characterize their roles in the physiology of plants. To characterize the subcellular localization and regulation of the transport proteins, we use confocal imaging technology. We also develop and deploy genetically encoded fluorescent biosensors for ions, metabolites and signaling molecules in vivo. We pioneered the development of transporter activity sensors. To be able to record sensor output, we require highly sensitive and quantitative fluorescence imaging technologies. In my previous lab at Stanford, we used both spinning disk and selective plane illumination microscopy to study transporter regulation and biosensor responses. This application requests funds for a set of two fluorescence microscopes as well as an image data storage and processing system. After careful testing and evaluation of multiple instruments, we would like to acquire: [...] The systems will not only be used by our team, but integrated into CAi (Center for Advanced Imaging) in Düsseldorf, and thus be accessible to a wide range of scientists. Moreover we have included specific descriptions of the needs for 5 additional teams at the University and the Clinics.