Spatial and temporal cellular assembly of the glycopeptide biosynthetic complex (B01+)

Balhimycin是由balhimycamycolatopsis合成的糖肽，是一种经过环化、甲基化、卤化和糖基化修饰的七肽。利用遗传学和生物化学，其生物合成步骤已被阐明，表明存在一个大的多酶复合物，催化氨基酸组装以及各种修饰。虽然可以确定反应的时间顺序，但对于这种超级复合物的位置和动力学却一无所知。蛋白质相互作用研究和最先进的显微镜技术将使复合物在细胞中定位，确定进一步可能的相互作用伙伴，并确定复合物的时间组装。

Balhimycin, a glycopeptide synthesized by Amycolatopsis balhimycina, is a heptapeptide, which is modified by cyclization, methylation, halogenation, and glycosylation. Using genetics and biochemistry, its biosynthetic steps have been elucidated indicating the occurrence of a large multi-enzyme complex that catalyzes amino acid assembly as well as various modifications. Whereas the chronology of the reactions could be defined, nothing is known about the positioning and dynamics of such a super-complex. Protein interaction studies and state-of-the-art microscopy will localize the complexes in the cell, identify further putative interaction partners, and define the temporal assembly of the complex.