Nanoscale organization of adaptor scaffolds involved in polarized cargo sorting at the trans-Golgi network (TGN) (A25*)

在项目A25中，我们将研究跨高尔基网络(TGN)的亚隔室纳米结构域组织，这可能是不同货物到不同细胞器或质膜结构域的时空控制分选的基础。利用CRISPR/Cas9敲入细胞系进行活细胞超分辨率成像和急性蛋白质降解策略，我们的目标是剖析基于适配器的膜支架蛋白在TGN纳米结构域组织中的功能作用，以分离和分类极化上皮细胞中不同的膜货物。

In project A25 we will investigate the sub-compartmental nanodomain organization of the trans-Golgi network (TGN) that likely underlies the spatio-temporally controlled sorting of distinct cargos to different organelles or plasma membrane domains. Harnessing CRISPR/Cas9 knock-in cell lines for live-cell super-resolution imaging and acute protein degradation strategies, we aim to dissect the functional role of adaptor-based membrane scaffolding proteins in TGN nanodomain organization to segregate and sort distinct membrane cargoes in polarized epithelial cells.