Microscale devices for in situ magnetic resonance enhanced by parahydrogen induced hyperpolarization - µDIMREPHiP

监测具有分子特异性的化学过程的能力在许多需要运输、化学转化和反应动力学的细节的学科中具有吸引力。 揭示生物系统中的这些细节在确定功能失调表型的发展及其对外部压力的反应的背景下是重要的。 在理解化学反应器内的反应进程和均匀性的背景下，技术系统也将受益于了解这些细节。 所面临的挑战是选择一种适当的分析技术，满足以下要求：非侵入性、非破坏性、广泛的化学和物理覆盖范围、高灵敏度和选择性。 磁共振（MR）是一种满足四个要求中的三个要求的技术。 它是非侵入性的，非破坏性的，并显示了广泛的适用性，测量物理和化学参数。 灵敏度和选择性是MR面临的主要挑战;然而，通过使用信号增强策略，这是可以克服的。 µDIMREPHiP团队的目标是开发能够通过同时满足四个所需分析功能来揭示化学过程细节的MR系统。项目成果将有可能对化学反应监测产生重大影响。 随着与NMR兼容的专用小型气液接触器的开发，PHiP之外的新分支应用将变得可用（例如，用于培养基pH值维持和充氧的气体供应，超极化惰性气体实验）。 设计用于通过NMR监测化学反应的微流体平台将对需要i）小规模、高通量筛选以优化反应和ii）使用生物系统进行快速药物筛选和作用模式研究的合作伙伴感兴趣。

The ability to monitor a chemical process with molecular specificity is appealing across many disciplines where details of transport, chemical conversion, and reaction kinetics are desired. Revealing these details in biological systems is important in the context of determining the development of dysfunctional phenotypes and their response to external stress. Technical systems also stand to benefit from knowing these details in the context of understanding reaction progress and homogeneity within chemical reactors. The challenge is in choosing an appropriate analytical technique satisfying the following requirements: non-invasive, non-destructive, broad chemical and physical coverage, high-sensitivity and selectivity. Magnetic resonance (MR) is a technique that addresses three of the four requirements. It is non-invasive, non-destructive and shows a broad applicability for measuring physical and chemical parameters. Sensitivity and selectivity is the primary challenge facing MR; however, by using signal enhancement strategies this can be overcome. It is the aim of the µDIMREPHiP team to develop MR systems capable of revealing the details of chemical processes by simultaneously satisfying the four desired analytical features. The project outcomes will have the potential to significantly influence chemical reaction monitoring in general. With the development of dedicated miniaturized gas-liquid contactors compatible with NMR, a new branch of applications will become available beyond PHiP (e.g. gas supply for pH maintenance and oxygenation of culture media, hyperpolarized noble gas experiments). Microfluidic platforms designed for chemical reaction monitoring by NMR will be interesting to partners requiring i) small-scale, high-throughput screening for reaction optimization, and ii) rapid drug screening and mode of action studies using biological systems.