Elucidating protein-RNA interactions in circRNA biogenesis and function (A01)

环状RNA(CircRNAs)是通过反向剪接产生的，在许多人类组织中大量表达，但它们在生理和疾病中的作用仍不清楚。该项目的目的是确定和表征CircRNA在内皮细胞中的生物发生和功能。具体地说，我们将通过生物信息学、生化方法和生理测定相结合的方法，研究先前发现的CircRNA cZNF292的功能和作用机制，并阐明CircRNA生物发生的调控。

Circular RNAs (circRNAs) are generated by backsplicing and are abundantly expressed in many human tissues, yet their contribution to physiology and disease remains largely unknown. The aim of this project is to identify and characterise circRNA biogenesis and functions in endothelial cells. Specifically, we will study the function and mechanism of action of the previously identified circRNA cZNF292 and elucidate the regulation of circRNA biogenesis by combining bioinformatics with biochemical approaches and physiological assays.