Physiological and Morphological Studies on Characteristics of Nociceptive Primary Afferent Neurons (Polymodal Receptors).

1. 为了阐明炎症过程中多模态受体活性的变化，采用体外睾丸精索神经制剂研究了炎症介质对睾丸多模态受体热反应的影响。前列腺素对热反应的增强作用是慢激素（BK）的100倍。5-HT在相同浓度范围内增强了这两种反应。组胺具有不同的作用：抑制或增强BK反应取决于组胺是否引起放电速率的显著增加，而组胺总是增强热反应。BK增加了热响应。这些介质都不能像55^ C-30s热刺激那样增强热反应。BK反应是由B2型受体介导的。2. 通过电镜可明确所鉴定睾丸多模态受体的外周结构。通过细胞内注射PHA-L，可追踪到所鉴定睾丸多模态受体的脊髓终止主要发生在I层。荧光双标记显示，支配睾丸的DRG神经元90%以上含有CGRP。3. 炎症介质引起的眼压变化的神经成分已得到澄清，但P物质作为其神经介质的可能性尚不支持。

1. In order to clarify changes in polymodal receptor activities in inflammation, effects of inflammatory mediators on the heat response of testicular polymodal receptors were studied in vitro using testis-spermatic nerve preparations. ProstaglandinE_2 augmented the heat responses from the concentration 100 times higher than that needed to augment the Bradykinin (BK) response. 5-HT augmented both responses in the same range of concentration. Histamine (His) had different effects : His suppressed or augmented the BK response depending on whether His induced substantial increase in discharge rate or not, whereas it always augmented the heat response. BK augmented the heat response. None of these mediators could augment the heat response so strong as 55^ﾟC-30s heat stimulation did. BK response was revealed to be mediated by B2 type receptor. 2. Peripheral structure of identified testicular polymodal receptors was clarified by EM. Spinal termination of identified testicular polymodal receptors was traced mainly in lamina I by intracellular injection of PHA-L. Double labeling with fluorescent dyes revealed that more than 90% DRG neurons innervating the testis contained CGRP. 3. Nervous component in changes in intraocular pressure induced by inflammatory mediators was clarified, but the possibility for substance P to be its neuronal mediator was not supported.