Isolation of cDNA Clone Encodes Monoclonal Nonspecific Suppressor Factor (MNSF)

我们从小鼠T细胞杂交瘤上清中获得MNSF。利用抗MNSF单克隆抗体分离MNSF，然后通过反相高压液相色谱（HPLC）进一步纯化。用全自动氨基酸序列分析仪分析MNSF。N-末端氨基酸序列数据显示与任何已知的生物活性蛋白包括非特异性抑制因子没有同源性，不幸的是，序列数据包括几个模糊的残基。MNSF的自聚集也使得难以分离cDNA。利用聚合酶链反应（PCR）制备MNSF基因的研究正在进行中。

We obtained MNSF from supernatant of murine T cell hybridoma. MNSF was isolated with the use of anti-MNSF monoclonal antibody, following which it was further purified by reverse-phase high pressure liquid chromatography (HPLC). MNSF was analyzed by auto amino acid sequence analyzer. The N-terminal amino acid sequence data showed no homology to any known biologically active proteins including nonspecific suppressor factor.Unfortunately, the sequence data include several ambiguous residues. Self-aggregation of MNSF also makes it difficult to isolate the cDNA. Further studies are under way to prepare MNSF gene with the use of polymelase chain reaction (PCR).