Study for Extensive Application of Salvaging Autotransfusion

研究一：将丝裂霉素C以5个浓度的15种组合作用于多种肿瘤细胞，暴露时间为3个，确定了抑制肿瘤细胞植入完整动物体内的最低暴露条件。对Ehrlich‘腹水瘤(EL-BP-3)的最低暴露条件为(1)100 mg/ml作用15分钟，(2)对Harding-Passey黑色素瘤作用15分钟，(3)对AH-66或AH-130大鼠肝癌作用20分钟。研究2：钙拮抗剂对抑制作用的相加作用。在研究1中获得的移植中，维拉帕米加入丝裂霉素C，使艾氏腹水瘤的最低丝裂霉素C浓度从100降至50微克/毫升，作用15分钟，但对Harding-Passey黑色素瘤无影响。研究3：其他抗癌药物的抑制作用。在BDF小鼠体内检测阿霉素、色霉素、阿克拉阿霉素和卡介苗对Harding-Passey黑色素瘤细胞移植的抑制作用。最低暴露浓度分别为：阿霉素10 mg/ml作用20分钟，色霉素500 mg/ml作用15分钟，阿克拉霉素1 mg/ml作用20分钟。即使当浓度增加到可溶的限度时，卡酚仍无任何作用。这一结果鼓励我们在手术中可以使用挽救的自体输血来切除恶性肿瘤，而红细胞则应用抗癌药物处理。

Study 1 : Various malignant cells were exposed to mitomycin C in vitro at 15 combinations of 5 concentrations and 3 exposure duration and minimum exposure condition which is capable to suppress implantation of tumor cells into intact animals was determined. The minimiim exposure condition was(1)100 mug/ml for 15 minutes against Ehrlich'ascites tumor(EL-BP-3), (2)400 mug/ml for 15 minutes against Harding-Passey melanoma, (3)100 mug/ml for 20 minutes against either AH-66 or AH-130 rat hepatoma.Study 2 : Additive effect of Ca-antagonist to the suppressive effects. in transplantation obtained in Study 1. Verapamil of 10 muM/ml added to mitomycin C decreased the minimum concentration of mitomycin C from 100 to 50 mug/ml for 15 minutes for Ehrlich'ascites tumor but not Harding-Passey melanoma. None of the additive effect was noted with nicardipine(at 10 - 100 muM/ml)for the tested tumor either.Study 3 : Suppressive effects of other anticancer drugs.The suppressive effects on the implantation of Harding-Passey melanoma cells with doxorubicin, chromomycin, aclarubicin and carboquone were tested in BDF mice. The minimum exposure concentration was 10 mg/ml for 20 minutes with doxorubicin, 500 mug/ml for 15 minutes with chromomycin and 1 mg/ml for 20 minutes with aclarubicin. None of the effect was noted with carboquone even when the concentration was increased up to the solvable limit of carboquone.These results encouraged us that salvaging autotransfusion could be used during operation for removal of malignant tumor, whereas the red cells should be treated with the anticancer drug.