Heart preservation and immunological tolerance induction in orthotopic heart transplantation in swines.

在各种全异基因小鼠组合中进一步研究环磷酰胺诱导的免疫耐受，同时尝试通过体外循环在猪体内建立原位心脏移植系统。给受体小鼠静脉注射100µg抗Thy 1.2单抗。第1，9×10^7全异基因脾细胞+3×10^7骨狭窄细胞静脉注射。第0天，环磷酰胺200 mg/kg，第2天，在不同的完全同种异体小鼠组合中，如C3H(H-2^k)*B6(H-2^b)、B6*C3H、BALB(H-2^d)*B6和BALB*C3H，诱导对耐受株的长期皮肤移植耐受。中位生存期分别为123天(n=19)、136天(n=9)、503天(n=15)和165天(n=8)。选用母猪(20-25公斤)。采用体外循环进行原位心脏移植。供心用九州大学停搏液(CP；Na87，K20)停搏，然后用CP或Collins‘s液(C；Na10，K117)冲洗并露出。保存温度为0ﾟC，保存4h。升主动脉开放后1小时，测定Emax。每组5头猪。CP组有3例(60%)和C组有1例(20%)能够停止体外循环。CP组3例中只有1例可进行Emax检查。LOS和出血是主要并发症。目前还没有找到任何长期幸存者。

Cyclophosphamide-induced immunological tolerance was further studied in various fully allogeneic murine combination, while a system of orthotopic heart transplantation was tried to established in swines by using cardiopulmonary bypass.Exp.1. When a recipient mouse was given 100mug anti Thy 1.2mAb i.v. on day-1,9x10^7 fully allogeneic spleen cells plus 3x10^7 bone narrow cells i.v. on day 0, and 200mg/kg cyclophosphamide on day 2, a long-lasting skin graft tolerance to the tolerogenic strain was induced in various fully allogeneic murine combinations such as C3H(H-2^k)*B6(H-2^b), B6*C3H, BALB(H-2^d)*B6, and BALB*C3H. The median survival (days) was 123 (n=19), 136(n=9), >503(n=15), and >165(n=8), respectively.Exp.2. Female swines (20-25kg) were used. Orthotopic heart transplantation was per-formed using cardiopulmonary bypass. The donor heart was arrested with Kyushu University cardioplegic solution (CP;Na87,K20)and then flushed with and emerged in the CP or in Collins' solution (C;Na10,K117). The preservation was performed at 0ﾟC for 4 hours. One hour after the aortic declamping, Emax was examined. Each group contained 5 pigs. Cardiopulmonary bypass was able to discontinue in 3(60%) in the CP group and in 1(20%) in the C group. Only one of the 3 of the CP group could be examined for Emax. LOS and bleeding was the major complications. Any long-term survivors were not obtained.