Studies on the cell-binding immunoglobulin M in human plasma

用亲和层析法从正常人血清中分离出一种免疫球蛋白M。这种免疫球蛋白M与谷氨酰胺类似物DON结合具有杀瘤活性。采用亲和层析法从正常人血清中分离出一种具有抗-半乳糖特异性的免疫球蛋白G。发现该免疫球蛋白的糖特异性不同于已知的抗半乳糖抗体，表明该免疫球蛋白G已经发育，并测定了7例癌症患者和50例正常人血清中该免疫球蛋白G的浓度。一名癌症患者血清中的浓度明显高于正常受试者。由于细胞表面末端β -半乳糖基的表达与肿瘤细胞的转移能力密切相关，因此该免疫球蛋白可能在癌症研究的各个领域具有潜力。

A kind of immunoglobulin M was isolated from normal human serum by affinity chromatography on an elastin-Sepharose column. This immunoglobulin M has a tumouricidal activity in combination with an glutamine analogue, DON.A kind of immunoglobulin G with anti-beta-galactosyl specificity was isolated from normal human serum by affinity chromatography on an lactose-Sepharose column. The sugar specificity of this immunoglobulin was found to be different from that of the known anti-galactosyl antibodies, indicating that this immunoglobullin G was developed, and the concentration of this immunoglobulin G in sera from 7 cancer patients and 50 normal subjects were determined. The concentration in the serum from one cancer patients was found to be markedly high as compared with that of normal subject. Since expression of the terminal beta-galactosyl groups on the cell surface is closely associated with metastatic ability of tumor cell, this immunoglobulin might have potential in various fields of cancer research.