Medico-Legal Study on the Systematic and Microscopic Procedure for Wound Examination

伤口检查在法医病理学中是最重要的，法医病理学家经常被要求评估伤口是如何造成的以及用哪种武器造成的。此外，与死亡时间一样，客观地确定伤口是死前造成还是死后造成一直是尸检的主要问题，如果是死前造成，则是死前多长时间造成的。本研究的目的是建立一套显微的、系统的伤口检查程序，以发现和记录各种类型的伤口，并更准确、客观地估计皮肤伤口的年龄。显微创面检查程序的法医学研究运用双目手术显微镜对法医尸检病例中的人体皮肤创面进行了显微观察。因此，使用手术显微镜进行伤口检查在确定擦伤的冲击方向，区分割伤和切口伤口等方面特别有用。更重要的是，刀锋损伤的刀伤两端和单刃刀钝面之间，旧疤痕和血管病变的密切观察，以及法医病理学家和临床医师的培训。损伤后皮肤创面年龄估计的实验研究本研究通过与I型胶原、纤维连接蛋白等细胞外基质的比较，阐明il -1 α的免疫组化定位，并探讨il -1 α定位随时间的变化是否可以为创面年龄的估计提供进一步的信息。因此，当需要确定损伤后3小时至1天的伤口年龄，即伤口愈合的急性炎症期时，免疫组织化学鉴定白细胞介素1 α定位似乎是非常有用的。并且，I型胶原和纤维连接蛋白在伤口愈合的增殖和成熟阶段也是有用的标记物。通过免疫染色il -1 α以及I型胶原和纤维连接蛋白，似乎可以更准确、客观地估计皮肤伤口的年龄。少

Wound examination is of prime importance in forensic pathology, and forensic pathologists are often required to assess how a wound was made and with which type of weapon. Furthermore, like the time of death, it is always main problem at autopsy to determine objectively whether a wound was ante-mortem or post-mortem, and if ante-mortem, how long before death it had been sustained. The purpose of this study is establishment of a microscopic and systematic procedure for wound examination in order to detect and record wounds of various kinds, and also to estimate skin wound age more accurately and objectively.1.Medico-legal study on the microscopic wound examination procedureHuman skin wounds were microscopically observed by the use of a binocular operation microscope in forensic autopsy cases. In result, wound examination using the operation microscope is particularly useful in the determination of the direction of impact in abrasions, differentiation between lacerations and incised wound … More s and between the two ends of a stab wound damaged by the blade and the blunt side of a single-edge knife, the close observation of old scars and blood vessel lesions, and the training of forensic pathologists and clinical physicians.2.Experimental study on the estimation of the skin wound age after injuryThe study was performed to elucidate immunohistochemical localization of IL-1alpha through the comparison with that of extracellular matrix such as collagen type I and fibronectin, and to discuss whether tha analysis of IL-1alpha localization with the passage of time may provide further information for the wound age estimation. In result, it seems to be much useful to immunohistochemically identify interleukin 1alpha localization when the determination of wound age between 3 hours and 1 day after injury, that is, in acute inflammatory phase of wound healing is needed. And, collagen type I and fibronectin are also useful as markers in the proliferative and maturation phase of wound healing. It seems possible, by immunostaining IL-1alpha as well as collagen type I and fibronectin, to estimate skin wound age more correctly and objectively. Less