Analysis of the function of collagen genes of tooth germ by controlling them.

发育中的牙胚含有多种类型的细胞外基质，包括I型、III型和IV型胶原。然而，这些胶原蛋白在牙齿细菌中的发育功能尚不清楚。在本项目中，我们利用反义寡脱氧核苷酸（ASO）分析了牙胚胶原基因的形态学功能，ASO与这些胶原mRNA的互补结合。所得结果如下：1)在含有ⅰ型、ⅲ型或ⅳ型胶原的ASO培养基中培养的牙胚，与仅在BGJb培养基中培养的对照牙胚或添加随机序列寡脱氧核苷酸的对照牙胚相比，组织的分化和牙本质、牙釉质的形成延迟。2)在含ⅰ型或ⅲ型胶原的ASO培养基中培养的牙胚，牙袋形成不足，牙釉质器官内出现上皮包涵体，牙袋部分穿孔。3)在含IV型胶原ASO培养基中培养的牙胚中，牙本质和牙釉质的形成延迟，牙釉质器官颈袢形态异常。这些发现有助于理解牙胚中胶原蛋白的发育功能。

The developing tooth germs contain many kins of extracellular matrices including type I, III and IV collagens. The developmental function of these collagens, however, are not known in the tooth germs. In this project, we analyzed morphologically the function of collagen genes of tooth germ using antisense oligodeoxynucleotides (ASO) which bind complimentally to mRNA's of these collagens. The results obtained are as follows.1) In the tooth germs cultured in the medium containing either of ASO's for type I, III or IV collagen, the differentiation of tissue and the formation of dentin and enamel delayd comparing with control tooth germs cultured in BGJb medium only or that plus random sequence oligodeoxynucleotide. 2) In the tooth germs cultured in the medium containing either of ASO for type I or III collagen, the formation of dental sacs were insufficient, and the epithelial inclusion bodies in the enamel organs and the partial perforations of dental sacs were observed. 3) In the tooth germs cultured in the medium containing ASO fortype IV collagen, the formation of dentin and enamel delayd and the abnormal morphology of the cervical loops of enamel organs was observed. It is thought that these finding contribute to the understanding of developmental functions of these collagens in tooth germs.