Morphological Study of Bursa Fabricus of Bob White Quail

(1)Histological tsudy of Bursa fabricus：Bursa Fabricus basically consists of inner epithelium，lamina propria mucosa，submucosa whithin developted lymph follicles，and capsule。At 0day of hatching，size of the follicles was measured 100mum in small one，and the follicle cortex developed slightly.At2-3weeks of age，the size of the follicles became lager and 220mum，the follicle cortex developed fairly.At6weeks of age，the size of the follicles became lager than these of 4or 5weeks of age and 500mum，the follicle cortex developed and wave finding between cortex and medulla was observed.At7weeks of age，the size of the follicles became the largest one and 700mum，the follicle cortex developed well。At9weeks of age，the size of the follicles became smaller one and decreased to 400mum，the follicle cortex maintained the development。At11-12weeks of age，the size of the follicles became150-400mum，the decrease of the follicle cortex was observed。At 14weeks of age，the size of the follicles became300mum，the follicle cortex became thin.These findings suggest that the deep relationship between the growth of the quail and the development·decrease of the lymph follicles was recognized.(2)Immunohistochemical study：B-cells were identified in the follicle by immunohistochemistry.(3)Identification of lymocytes：Midle IymocytesThe cells with microvilli were identified B-cells by scanning electron microscopy.These findings suggest that the identification of the cells was possible by immunohistological methods and electron microscopy。

(1) Histological tsudy of Bursa fabricus :Bursa Fabricus basically consists of inner epithelium, lamina propria mucosa, submucosa whithin developted lymph follicles, and capsule. At 0 day of hatching, size of the follicles was measured 100mum in small one, and the follicle cortex developed slightly. At 2-3 weeks of age, the size of the follicles became lager and 220mum, the follicle cortex developed fairly. At 6 weeks of age, the size of the follicles became lager than these of 4 or 5 weeks of age and 500mum, the follicle cortex developed and wave finding between cortex and medulla was observed. At 7 weeks of age, the size of the follicles became the largest one and 700mum, the follicle cortex developed well. At 9 weeks of age, the size of the follicles became smaller one and decreased to 400mum, the follicle cortex maintained the development. At 11-12 weeks of age, the size of the follicles became 150-400mum, the decrease of the follicle cortex was observed. At 14 weeks of age, the size of the follicles became 300mum, the follicle cortex became thin.These findings suggest that the deep relationship between the growth of the quail and the development ・ decrease of the lymph follicles was recognized.(2) Immunohistochemical study :B-cells were identified in the follicle by immunohistochemistry.(3) Identification of lymphocytes :Middle Iymphocytes and mitosis were observed in the follicle cortex and small or large cells were observed in the follicle medulla by transmission electron microscopy. The cells with microvilli were identified B-cells by scanning electron microscopy.These findings suggest that the identification of the cells was possible by immunohistological methods and electron microscopy.