Immunological and cytochemical studies in the cytoplasmic bridge of reproductive cells.

在广泛的动物物种中，许多生精细胞是通过细胞质桥连接起来的。本研究的目的是阐明为什么具有狭窄细胞质连续性的特定桥结构对精子发生的先后进行是必要的。我们报道了肝半螺旋藻细胞质桥的质膜具有特定的膜构型；1)膜缺乏膜内蛋白和胆固醇，2)膜具有结合几种卵磷脂的糖萼，3)膜在盘状边缘呈波纹状，4)膜与周期性条纹的丝状束相关，5)由质膜和丝状束组成的桥复合物对皂苷和非离子表面活性剂Nonidet P-40具有抗性。本文的研究结果如下：1) Nonidet P-40能分离出桥状复合物，但桥状复合物的分离效果不足以产生免疫单克隆抗体；2)条状丝状束与盘状桥状膜周围的所有膜相结合，连接了三个生精细胞之间的两个桥状膜；3)使用Nonidet P-40分离后，能应用于乳腺生精细胞；(4)哺乳动物的生精细胞桥没有横纹束；(5)半针叶纲生精细胞的横纹束对肌动蛋白和10nm细丝没有免疫反应；(6)从生精细胞冻裂副本的显微照片密度测定来看，桥质膜比其他的更干净光滑。因此，桥质膜被认为由包括糖脂在内的饱和脂肪酸组成。许多克隆生精细胞主动控制它们与乳母细胞之间的桥的排列。

A number of spermatogenic cell in wide animal species are connected by cytoplasmic bridges. Purpose of this research is clarified the reason why the specific bridge structures with narrow cytoplasmic continuity are necessary to progress the spermatogenesis successively.We had reported the plasma membrane of cytoplasmic bridges has specific membrane configulations in Semisarcospira livertina ; 1) the membrane lacks intramembranous proteins and cholesterol, 2) the membrane has glycocalyces which conjugate several lectines, 3) the membrane corrugates around the discoidal edge, 4) the membrane is associated with periodically striated filamentous bundles, 5) the bridge complex which consists of the plasma membrane and filamentous bundles is resistant to saponin and to non-ionicsurfactant, Nonidet P-40.Results in the presn research were as follows ; 1) the bridge complex was able to be separated with Nonidet P-40, but the separation of bridge complexes was not good enough for the production of immunological monoclonar antibody, 2) the striated filamentous bundle were associated with all around the discoidal bridge membrane and connected two bridges between three spermatogenic cells, 3) the separation, using Nonidet P-40 were able to apply the mammarian spermatogenic cells, 4) the bridges of spermatogenic cells in mammal had not the striated bundle, 5) the striated bundles in Semisarcospira do not show immuno-reactivity to actin and 10nm filaments, 6) judging from micrographs' densitometry of the freeze fracture replica on the spermatogenic cells, bridge plasma membranes are cleanly more smooth than the other.Consequently, the bridge plasma membrane is thought to consist of saturated fatty acids including glycolipids.A number of clonal spermatogenic cells control actively their bridges' arrangement to the nurse cells.