Expression of neuron-specific B-tubulin protein in non-neoplastic and neoplastic tissues.

利用单克隆抗体TuJ1检测了神经元特异性分类β同型微管蛋白在几种产生胺肽的内分泌肿瘤中的表达发生率。61例垂体腺瘤中有43例（73%）表达这种同型。这表明微管蛋白同型的异位表达。100%的肾上腺嗜铬细胞瘤、45%的甲状腺髓样癌、30%的胰腺内分泌肿瘤、25%的类癌和10%的肺小细胞癌表达微管蛋白同型。使用相同的抗体TuJ1，通过免疫染色检测了不同发育阶段鸡鳃末梢腺中神经元特异性β -微管蛋白同型的定位。孵育8天后，许多C细胞被强烈染色以确定同型。染色在胚胎发育后期开始减少。孵化后，染色变弱，并且在10天大的鸡中，仅限于鳃末梢腺的神经纤维。采用同种抗体TuJ1，采用免疫组化方法观察不同发育阶段鸡颈动脉小体和颈总动脉管壁血管球细胞群的发育情况。从培养第9天开始，免疫反应细胞继续与迷走神经节远端开始进入颈动脉体区。在12天的孵卵期，身体的大部分被免疫反应性细胞占据。本研究表明，颈动脉体中的血管球细胞是从迷走神经节远端到达每个居住地的移植物。电镜免疫细胞化学显示，该亚型定位于非肿瘤周围神经的神经小管，而不定位于神经元细胞的其他部位。

The incidince of expression of neuron-specific ClassIIIbeta isotype tubulin was examined by use of a monclonal antibody TuJ1 in several types of amine-peptide producing endocrine tumors. Forty-three out of 61 pituitary adenomas (73%) expressed this isotype. This indicated an ectopic expression of the tubulin isotype. 100% of adrenal pheochromocytomas, 45% of medullary thyroid carcinomas, 30% of pancreatic endocrine tumors, 25% of carcinoid tumors, and 10% of pulmonary small cell carcinomas expressed the tubulin isotype.The localization of the neuron-specific beta-tubulin isotype in the ultimobranchial glands from chickens at various stages of development was examined by immunostaining, using the same antibody TuJ1. Many C cells were intensely stained for the isotype at 8 days incubation. The staining started to decrease at late stages of embryonic development. After hatching, the staining became weaker and, in 10-day-old chickens, restricted to the nerve fibers of the ultimobranchial gland.Development of the carotid body and the glomus cell groups in the wall of the common carotid artery was examined in chickens at various developmental stages by immunohistochemistry using the same antibody TuJ1. From 9 days of incubation, the immunoreactive cells continuing with the distal vagal ganglion began to enter into the carotid body anlage. At 12 days of incubation, a large portion of the body were occupied by the immunoreactive cells The present study demonstrates that the glomus cells in the carotid body are emigtres that arrive in each residential place from the distal vagal ganglion.The isotype was localized at neurotubules of non-neoplastic peripheral nerves but not localized in other sites of neuronal cells by electron microscopic immunocytochemistry.