Identification of HTLV-1 neutralization epiotpe amino acid sequence its and application

为了确定人t细胞白血病病毒i型（HTLV-I）包膜抗原的中和区，我们从猪和小鼠中获得了许多新的抗包膜gp46单克隆抗体。新单抗识别的表位定位于gp46氨基酸序列186-193,190-195,191-195,191-196和194-199，在合胞体和转化抑制试验中可以中和HTLV-I。卵清蛋白（OVA）偶联的合成gp46肽含有这些中和表位，氨基酸190-199 （pep190-199）和pep180-204，但不含pep185-194或pep194-203，可以在兔体内产生HTLV-I中和抗体反应。然后用5 × 10^7个活的HTLV-I产生物ILT-8M2细胞对这些免疫或非免疫的家兔进行HTLV-I攻击。聚合酶链反应（PCR）结果显示，在未免疫和pep288-312免疫家兔的PBL中检测到HTLV-I原病毒，而在较长时间内，在prp190-199和pep180-204免疫家兔的PBL中未检测到HTLV-I原病毒。这些结果表明，通过合成肽免疫诱导抗gp46中和抗体反应具有保护动物体内免受htlv - 1感染的潜力。

In order to define neutralization regions the envelope antigen of human T-cell leukemia virus type-I (HTLV-I) we have generated a number new antienvelope gp46 mAbs fromrats and mice. Epitopes recognized by new mAbs, which could neutralize HTLV-I in syncytium and transformation inhibition assays, were localized to gp46 amino acid sequences, 186-193,190-195,191-195,191-196 and 194-199. Ovalbumin (OVA) -conjugated synthetic gp46 peptides containing these neutralization epitopes, amino acids 190-199 (pep190-199) and pep180-204, but not pep185-194 or pep194-203, could give rise to HTLV-I neutralizing antibody responses in rabbits. These immune or non-immune rabbits were then challenged with HTLV-I by i. v. inoculation with 5x10^7 live HTLV-I producer ILT-8M2 cells. By a polymerase chain reaction (PCR) assay, it was revealed that HTLV-I provirus was detected in PBL from non-immune and pep288-312-immunized rabbits, whereas the provirus was not detected in PBL from the prp190-199 and pep180-204-immunized rabbits over an extended period. These results suggest that the induction of anti-gp46 neutralizing antibody responses by immunization with synthetic peptides has the potential to protect animals against HTLV-I infection in vivo.