A new serum maker for transitional cell cancer

针对移行细胞癌(TCC)的特异性和敏感性的单抗可能有助于肿瘤的早期发现和精确的治疗监测。我们研制了一种抗TCC的单抗(EH14)。在本项目中，我们试图纯化EH14识别的抗原。我们还比较了TCC患者和健康对照组血清和尿液中的抗原浓度。为了评价EH14阳性细胞和阴性细胞的特性差异，采用流式细胞术对TCC细胞进行检测。此外，我们正在从TCC细胞系中构建EH14抗原基因的cDNA文库，利用亲和层析法对EH14抗原进行部分纯化，并测定其氨基酸序列。用双抗体夹心ELISA法检测，TCC患者血清和尿液中EH14抗原浓度均显著高于健康对照组。有淋巴结转移患者的血清抗原滴度浓度高于无淋巴结转移患者，但差异无统计学意义。流式细胞仪检测显示DNA倍体和EH14染色模式因患者不同而不同。统计分析将在累积检查的病例后进行。

A specific and sensitive monoclonal antibody against transitional cell cancer (TCC) may be useful for early detection of the tumor and precise monitoring of the treatment. We have developed a monoclonal antibody (EH14) against TCC.In this program we tried to purify the antigen recognized by EH14. We also compared the antigen concentration in the serum and the urine of patients with TCC to healthy controls. In order to evaluate the difference of the characteristics between the EH14 positive and negative cells, the TCC cells were exmined by flow cytometry. In addition, we are making cDNA libraries from TCC cell lines to identify the EH14 antigen gene.Using an affinity coloumn EH 14 antigen was partially purified and the amino acid sequence is to be determined. Using a sandwich ELISA system, the serum and the urine concentrations of EH14 antigen in patients with TCC were significantly higher than tose in healthy controls. The patients with lymph node metastases had higher concentration of the serum antigen titer than tose without lymph node metastases although not statistically significant. Flow cytometric evaluation showed that the patterns of DNA ploidy and EH14 staining varied from patient to patient. The statistical analysis will be done after the accumulation of cases examined.