Establishment and characterization of T cell clones from autologous tumor-specific T cells in renal cell carcinoma

本研究利用白细胞介素2（IL 2）基因转染的小鼠肾癌细胞（Renca），建立肿瘤特异性自体T细胞，并对其进行鉴定。经CTLL-2生物测定和RT-PCR证实转染IL-2基因的Renca（Renca Neo/IL-2）能产生IL-2，并经Balb/c小鼠皮下移植瘤组织学检查显示，Renca Neo/IL-2组淋巴细胞浸润程度明显高于Renca Neo组。荷瘤Balb/c小鼠脾细胞和肿瘤浸润淋巴细胞（TIL）的细胞毒活性表明，Renca Neo/IL 2细胞的细胞毒活性明显高于Renca Neo细胞，但不具有自体肿瘤特异性。结论：Renca Neo/IL 2诱导肿瘤局部淋巴细胞浸润，比Renca Neo诱导的细胞毒淋巴细胞具有更强的靶向性和细胞毒活性。

The present study was designed to establish and characterize the autologous tumor-specific T cells with the use of interleukin 2 (IL 2) gene-transfected murine renal cell carcinoma (Renca). Production of IL 2 by IL 2 gene-transfected Renca (Renca Neo/IL 2) was confirmed by bioassay with CTLL-2 and by RT-PCR.Histological examination of subcutaneous tumors established in Balb/c mice showed that the extent of lymphocyte infiltration in Renca Neo/IL 2 was more significant than that in Renca Neo. Cytotoxic activity of splenic cells and tumor-infiltrating lymphocytes (TIL) from tumor (Renca Neo/IL 2 or Renca Neo)-bearing Balb/c mice revealed that cytotoxic activity of the cells from Renca Neo/IL 2 was significantly higher than that of Renca Neo but was not autologous tumor specific. In conclusion, Renca Neo/IL 2 induces lymphocyte infiltration in local tumor mass, and is more potent than Renca Neo with regard to inducing cytotoxic lymphocytes with wide target spectrum and high cytotoxic activity.