Effect and its intracellular distribution of calcium on poory motile spermot 20a.

用荧光显微镜下的荧光图像分析仪观察细胞内游离钙浓度的变化及在无钙或富钙培养基中的分布。在无钙培养液中稀释时，金黄地鼠精子线粒体再生区周围细胞内游离钙水平略有升高。相反，当在富钙培养基中稀释时，其水平显著增加。细胞外钙离子似乎是通过顶体再生来转运的。人精子运动良好时，细胞外钙离子不仅存在于顶体区，而且普遍存在于头部周围。然而，在运动不良的人精子，细胞外钙从来没有被纳入细胞内，即使钙提出了足够的细胞外。

Change in intracellular level and distribution of free-calcium following dilution in calcium-free or calcium-enriched medium were investigated using fluorescent image analyzer equipped with fluorescent microscope. When diluted in calcium-free media, intracellular free-calcium level elevated slightly around mitochondrial regeon in spermatozoa of golden hamster. On the contrary, the level increased significantry when diluted in calcium-enriched medium. The extracellular calcium seemed to be incorported via acrosomal regeon. In human well moved spermatozoa, the extra-cellular calcium was incorporated universally all around the head reageon, not remained only acrosomal regeon. However in poorly moved human spermatozoa, extracellular calcium was never incorporated intracellularly even though the calcium presented enough extracellularly.