ELECTRON MICROSCOPIC STUDY ON THE STROMAL STRUCTURE OF ORAL SQUAMOUS CELL CARCINOMA

采用兔VX2舌癌移植瘤模型，观察了舌癌和转移淋巴结中胶原纤维的形态变化，以及胶原酶和纤溶酶原激活物的酶活性变化。光镜观察发现，移植后7d舌癌生长迅速，间质细胞增多。Fastgreen和天狼星红染色观察表明，随着肿瘤的生长，胶原纤维逐渐消失。而颈深部淋巴结转移灶胶原纤维未见明显变化。扫描电子显微镜观察结合氢氧化钠浸渍法立体观察发现，舌癌中胶原纤维压缩…。淋巴结转移灶胶原纤维降解较多，构筑粗糙，提示胶原纤维已发生降解，移植后第14天，舌癌中心胶原酶活性达到高峰。移植后21d，外周血中多形核白细胞中胶原酶活性升高，移植后21d达到高峰。纤溶酶原激活剂在舌癌中心和边缘区域的活性呈时间依赖性增加。移植后血清中纤溶酶原激活物显著升高，移植后21d尿液中纤溶酶原激活物显著升高，说明胶原酶和纤溶酶原激活剂在体内表现出不同的动力学特征，但当两者相互作用时，两种酶都深入参与了胶原纤维的降解和溶解。此外，血液和尿液中胶原酶和纤溶酶原激活物活性的分析为估计肿瘤生长提供了有用的临床参数。较少

Using VX2 carcinoma transplanted into the tongue of domestic rabbits, morphological changes in the collagen fibers of the tongue tumor and metastatic lymph nodes as well as the kinetics of the enzymatic activities of collagenase and plasminogen activator were examined. The relation between these enzymes was analyzed with reference to degradation of collagen fibers in the tumor tissue, and the levels of these enzymes in the serum and urine.Light microscopic examination revealed that the tongue tumor grew rapidly 7 days after transplantation, associated with increased cells in the stroma. Observations using Fastgreen and Sirius-red stain indicated that collagen fibers gradually disappeared as the tumor grew. However, no marked changes were observed in the collagen fibers of metastatic foci in the deep cervical lymph nodes.Scanning electron microscopic observation used concurrently with the NaOH maceration method revealed stereoscopically that collagen fibers in tongue tumor were compress … More ed and degraded, and the architecture of the collagen tissue was coarse in the metastatic foci of the lymph nodes, suggesting that degradation of collagen fibers had occurred.Collagenase activity peaked in the center of the tongue tumor 14 days after transplantation. Collagenase activity in the polymorphonuclear leukocytes in peripheral blood increased and peaked 21 days after transplantation.Plasminogen activator exhibited a time-dependent increase in activity in the center and marginal regions of the tongue tumor. There was also a significant increase in plasminogen activator rapidly decreased in the serum after transplantation, but significantly increased in the urine 21 days after transplantation.These results indicate that collagenase and plasminogen activator show different kinetics, but when they interact, both enzymes become deeply involved in the degradation and lysis of collagen fibers. Furthermore, analysis of blood and urine for collagenase and plasminogen activator activity provides useful clinical parameter for the estimation of tumor growth. Less