DNAプローブ集積チップによる塩基配列の高速探査

DNA探针集成芯片是基于硅或玻璃的小表面，许多DNA寡核苷酸固定在其上。该芯片为基因组定位、SBH高通量测序、DNA诊断和基因表达谱分析等克隆排序提供了基础技术。在本研究中，我们开发了用于DNA探针集成芯片制作的光定向寡核苷酸固定化方法。光导寡核苷酸固定方法将预先合成的DNA寡核苷酸和PCR产物整合在固体载体上的指定位置。因此，它可以制造带有DNA探针的DNA探针集成芯片，长度足够精确地识别大型基因组中的特定基因。

DNA probe integrated chips are silicon-or glass-based small surfaces on which many DNA oligonucleotides are immobilized. The chips provide a basic technology to perform clone-ordering for genome mapping, high-throughput sequencing by SBH, DNA diagnosis and gene expression profile analysis. In the present study, we have developed the light-directed oligonucleotide immobilization method for making DNA probe integrated chips. The light-directed oligonucleotide immobilization method integrates presynthesized DNA oligonucleotides and PCR products at the addressed positions on solid support. So it can make DNA probe integrated chips with DNA probes long enough to identify a particular gene of a large genome precisely.