Alterations of opoptosis and cell cycle resulation induced by heptatitis Cvirus

肝癌的发生机制尚不清楚，HCV没有致癌基因，HCV基因的表达可能会改变细胞周期调控、诱导细胞凋亡等细胞机制。为了研究HCV蛋白对这些细胞调控系统的作用机制，我们尝试将HCV基因片段导入表达载体。HCV基因逆转录扩增。将产生的基因片段导入逆转录病毒表达载体（pZIPneo）。将携带HCV基因片段的逆转录病毒载体转染PA317包装细胞。然后，将这些细胞产生的逆转录病毒转导到人肝癌细胞中。采用免疫印迹和免疫荧光抗体技术分析HCV蛋白的表达。关于HCV基因表达对药物诱导的细胞凋亡和细胞周期进程的影响的研究正在进行中。

The mechanism of hepatoma evelopment is not known, HCV has no cancer genes and the expression of HCV gene is supposed to alter the cellular mechanisms such as cell cycle regulation and induction of apoptosis. To investigate the mechanism of the action of HCV protein on these cell reguratory system, we attempted to introduce fragments of HCV gene into expression vectors. The HCV gene were reverse transcribed and amplified. Resulting gene fragments were introduced into a retroviral expression vector (pZIPneo). The retroviral vector carrying HCV gene fragments was tansfected into PA317 packaging cells. Then, the retrovirus produced by these cells were transduced into human hepatoma cells. The expression of HCV proteins are analyzed by immuno-blotting and immunofluorescent antibody technique. The studies about the effects of the HCV gene expression on the drug-induced apoptosis and cell cycle progression are in progress.