Purification of a novel perforin Inhibitor

我们将确定如何将移植物的功能保存在储存液中，以及如何防止移植物发生急性和/或慢性排斥反应。但是，必须从新的角度和新的角度改进储存液。我们正在思考癌细胞与CTL和/或NK细胞之间的关系。一般说来，癌细胞可以逃脱免疫细胞的攻击。我们证实癌细胞有很强的穿孔素抑制剂，并远离这些免疫细胞。我们有许多无蛋白培养的癌细胞系(66个系)，并制定了从这些细胞系及其条件培养液中纯化穿孔素抑制物的计划。根据我们的计划，我们可以从细胞裂解液和HGC-Y1和HPC-Y3的条件培养液中纯化出强的穿孔素抑制物。N-末端氨基酸序列分析显示PEPAKSA PAPKKGSKKAVTK。令人惊讶的是，该序列与人类组蛋白H_2B完全相同。事实上，小牛组蛋白具有很强的穿孔素抑制活性。根据这些结果，在移植物储存液中注入组蛋白并将该抑制剂注射给发生急性排斥反应的受体将是非常有用的，因为大量给予正常大鼠小牛组蛋白从未在任何大鼠器官中引起任何异常的组织学发现。

We are going to establish how to keep the funtion of the graft in the storage fluid, and how to prevent the acute and/or chronic rejection of the graft from recipient. However, it is necessary to Improve the storage fluid from fresh and new stand points. We are thinking about the relationship between cancer cells and CTL and/or NK cells. In general, cancer cells escape from the attack of these immune cells. We confirm that cancer cells have strong perforin inhibitor, and get away from these immune cells. We have many protein-free cultured cancer cell lines (66 lines), and made a plan to purify the perforin inhibitor from the cell lines and their conditioned media. According to our plan, we could purified strong perforin inhibitor from cell lysate and conditioned medium of HGC-Y1 and HPC-Y3. The analysis of N-terminal amino acid sequences showed PEPAKSA PAPKKGSKKAVTK. Surprisingly, this sequence was perfectly identical to human histone H2B. Indeed, calf histone has very strong perforin inhibitor activity. From these results, the administration of histone protein in the graft storage fluid and the injection of this inhibitor to the recipient with acute rejection will be very useful, since a large giving of calf histone to normal rats never caused any abnormal histological findings in any rat organs.