REGULATION MECHANISM BETWEEN CELL-MEDIATED AND HUMORAL IMMUNE RESPONSE TO MM-ANTIGEN

我们已经报道了与MM Tumor Cell一起使用L CELLS的Somatic Tumor Hybrid的L Cell如何孤立地失败到Induce细胞（CTL），但反体到MM ANTIGEN在Syngenic MICE，可能印度MM-Specific CTL和那与L Cell释放杀手-HELPER Factor的互动的未Primed Syngenic Spleen Cellls（S） Which Plays在CTL一代的Important Rolle，和Mouse Beta-Interferon （IFN）可以替代它。The Following Resorts Were Obtained IN A Series OF Experiences（I.）Killer-Helper Effect OF Mouse Beta-IFN IN THE Secondary CTL Generation IN Vitro; Maximal MM-Specificic CTL （Thy-<1^+> ，LYT-<2^+> ）Generation is Observed IN THE Presence OF IFN During THE First 24-HR文化和IFN的效果是由Specificanty-IFN抗体完成的。Macrophages IN Responder Cell流行是IFN行动的目标。二. KILLER HELPER FACTOR（S）; ISOLATION OF THE FACTOR（S） WAS UNSUCCESSFUL（S）CSF的活动， IFN和IL-3， Except IL-2是在细胞-自由文化超级国民加入杀手-帮助因子（S），但只有IFN活动Seemed TO BE Related WITH Helper ACTIVITY。Gamma-IFN是由于PH 2治疗而导致的活动损失的令人怀疑的原因，但老鼠回收的gamma-IFN是作为杀手-助手因素。三.三. L细胞在反人体反应上的效果;在Helper T细胞在反人体反应之间没有差别;在Helper T细胞的作用中，反人体反应在MM Tumor和混合细胞之间。四.杀手-助手在初级CTL一代中的L细胞效应： CTL不是由加热引起的（47英寸， 60分钟）的芳香细胞在缺乏但存在的L细胞。因子包含文化超级大国或更好-IFN也在这个系统中展示帮助者活动，并在CTL一代的早期步骤中采取了行动。阿亚洛的复兴-<GM 1 ^+>细胞从反应者流行失败到印度所有-CTL甚至在L细胞的存在。

WE HAVE REPORTED THAT SOMATIC TUMOR HYBRID OF L CELL WITH MM TUMOR CELL WHICH ALONE FAILED TO INDUCE CYTOTOXIC T CELLS (CTL) BUT ANTIBODIES TO MM ANTIGEN IN SYNGENEIC MICE, COULD INDUCE MM-SPECIFIC CTL AND THAT INTERACTION OF UNPRIMED SYNGENEIC SPLEEN CELLS WITH L CELLS RELEASED KILLER-HELPER FACTOR(S) WHICH PLAYS AN IMPORTANT ROLE IN CTL GENERATION, AND MOUSE BETA-INTERFERON (IFN) COULD SUBSTITUTE IT. BASED ON THESE RESULTS, THE FOLLOWING RESULTS WERE OBTAINED IN A SERIES OF EXPERIMENTS: I. KILLER-HELPER EFFECT OF MOUSE BETA-IFN IN THE SECONDARY CTL GENERATION IN VITRO; MAXIMAL MM-SPECIFIC CTL (THY- <1^+> , LYT- <2^+> ) GENERATION WAS OBSERVED IN THE PRESENCE OF IFN DURING THE FIRST 24-HR CULTURE AND THE EFFECT OF IFN WAS COMPLETELY INHIBITED BY SPECIFIC ANTI-IFN ANTIBODY. MACROPHAGES IN RESPONDER CELL POPULATION WERE TARGETS FOR ACTION OF IFN. II. KILLER-HELPER FACTOR(S); ISOLATION OF THE FACTOR(S) WAS UNSUCCESSFUL. ACTIVITIES OF CSF, IFN AND IL-3, EXCEPT IL-2 WERE PRESENT IN CELL-FREE CULTURE SUPERNATANTS CONTAINING KILLER-HELPER FACTOR(S), BUT ONLY IFN ACTIVITY SEEMED TO BE RELATED WITH HELPER ACTIVITY. GAMMA-IFN WAS SUGGESTED BECAUSE OF LOSS OF ACTIVITY BY PH 2 TREATMENT, BUT MOUSE RECOMBINANT GAMMA-IFN WAS INACTIVE AS KILLER-HELPER FACTOR. III. EFFECT OF L CELLS ON ANTIBODY RESPONSE; THERE WAS NO DIFFERENCE IN THE CAPACITY FOR HELPER T CELL INDUCTION IN ANTI-TNP ANTIBODY RESPONSE BETWEEN MM TUMOR AND HYBRID CELLS. IV. KILLER-HELPER EFFECT OF L CELLS IN THE PRIMARY CTL GENERATION: CTL WERE NOT PODUCED BY STIMULATION WITH HEATED (47゜, 60 MIN) ALLOGENEIC CELLS IN THE ABSENCE BUT PRESENCE OF L CELLS. THE FACTOR CONTAINING CULTURE SUPERNATANT OR BETA-IFN ALSO SHOWED HELPER ACTIVITY IN THIS SYSTEM AND ACTED AT THE EARLY STEP OF CTL GENERATION. REMOVAL OF ASIALO- <GM1^+> CELLS FROM THE RESPONDER POPULATION FAILED TO INDUCE ALLO-CTL EVEN IN THE PRESENCE OF L CELLS.