Pathological study of hemorrhagic fever with renal syndrome virus among experimentally infected rats.

本研究旨在通过免疫组织学技术揭示肾综合征出血热相关病毒的致病性、病毒的传播方式以及大鼠对病毒的敏感性。因此，用SR-11毒株对新生大鼠和成年大鼠进行了实验感染。用ABC方法在这些动物的不同器官中检测到病毒抗原。在初步研究中，对SR-11株感染的Vero E-6细胞进行了免疫电子显微镜检查。病毒抗原存在于细胞质中的包涵体和小泡中，但在这些结构中未观察到病毒颗粒。从这项研究中，包涵体被认为是病毒增殖的副产品。乳鼠(<24小时)经腹膜腔内接种。用SR-11菌株的10^3 FFU进行路线选择。他们表现出中枢神经症状，如瘫痪和痉挛，并在四周后死亡。…在肺、脑、肾、脾、肝、心等组织中检测到病毒抗原唾液腺较多，但肠道内未检测到抗原。乳鼠和成年大鼠均经腹腔接种。或经鼻途径接种sr-11毒株，根据抗体产量计算50%感染剂量(ID_&lt；50&gt；)。不同年龄组或接种途径的ID_&lt；50&gt；值无显著差异。给哺乳大鼠腹腔注射。SR-11毒株免疫途径中，抗原和抗体的阳性率随接种剂量的增加而增加。而鼻腔接种乳鼠和成年大鼠经鼻腔接种病毒后，肺组织中的病毒抗原含量较低。只有在注射高剂量病毒后才能使用。虽然观察到抗体的产生，但在任何器官中都没有检测到病毒抗原。用感染乳鼠饲养的4只乳鼠中，有2只出现抗体转换。此外，在血清转换动物的肺组织中也检测到病毒抗原。较少

This study was undertaken to reveal pathogenicity of HFRS related virus, the mode of transmission of the virus and the sensitivity of rats to the virus by an immunohistological technique. Accordingly, neonatal and adult rats were Experimentally infected with SR-11 strain. Viral antigen was detected in various organs from those animals by ABC methods.1. In the preliminary study, infected Vero E-6 cells with SR-11 strain was examined by immuno-electron microscopical procedures. Viral antigen was detected in inclusion bodies and small vesicles in the cytoplasm, however, viral particles were not observed in these structures. From this study, the inclusions are considered to be by-products of viral proliferation.2. Suckling rats (less than 24 hr) were inoculated by intraperitoneal (i.p.) route with 10^3 FFU of SR-11 strain. They showed central nervous symptoms, such as paralysis and convulsion, and died by four weeks. Viral antigen was detected in lung, brain, kidney, spleen, liver, heart a … More nd salivary gland for a long time, but no antigen was detected in intestine.3. Suckling rats and adult rats were inoculated by i.p. or nasal route with sr-11 strains and 50% infectious dose (ID_<50>) were calculated according to production of the antibodies. The ID_<50> values in contrasting age groups or inoculation routes did not show any significant differences.4. Suckling rats inoculated by i.p. route with SR-11 strain showed increasing positive rates in both antigen and antibody depending on the increase of recieving viral dose. However, viral antigen was limited in the lungs from suckling rats inoculated by nasal route and adult rats inoculated by i.p. route only after administration of high viral dose. Although the production of antibodies was observed, no viral antigen was detected in any organs.5. Antibody conversions were observed in 2 of 4 suckling rats which were kept with infected suckling rats. Furthermore, viral antigen was detected in the lung tissues from the sero-converted animals. Less