The effects of antineoplastic agent and irradiation on Ga-67 uptake in tumor cells

1. (1)本实验旨在研究Ga-67的摄取与辐照及抗肿瘤药物对肿瘤细胞的作用之间的关系，得到以下结果：1000 rad、2000 rad和3000 rad辐照均能抑制动物肿瘤的生长，1000 rad、2000 rad和3000 rad辐照使动物肿瘤对Ga-67的摄取减少，肿瘤组织在辐照下表现出明显的变性和坏死。抗肿瘤药物阿霉素对培养的肿瘤细胞（小鼠白血病细胞：L5178Y）的生长有抑制作用。然而，添加阿霉素后，培养的肿瘤细胞对Ga-67的摄取增加。(2)研究肿瘤细胞Ga-67摄取与ATP代谢的关系。研究了ATP代谢抑制剂NaF和Ouabain对培养细胞Ga-67摄取的影响。与对照组相比，NaF和瓦巴因处理的细胞对Ga-67的摄取减少。(3)研究了Ga-67摄取与肿瘤细胞骨架丝的关系。添加细胞松弛素B后，肿瘤细胞对Ga-67的摄取明显减少。发现细胞骨架丝在肿瘤细胞对Ga-67的摄取中起重要作用。临床试验经化疗有效照射后，发现肿瘤中Ga-67的积累减少。为了进一步探讨这一问题，我们研究了颈部恶性肿瘤患者放疗化疗后Ga-67闪烁图的变化。所有恶性肿瘤患者治疗前Ga-67显像均显示阳性。然而，经过有效治疗后，Ga-67闪烁图变为阴性。这一观察结果表明，Ga-67闪烁图可以提供有关治疗效果的有用信息。

1. Experimental research(1) The purpose of this investigation was undertaken in order to study the relationships between the Ga-67 uptake and effect of irradiation and antineoplastic agent on The tumor cells, and following results were obtained. The growth of animal tumor was inhibited by irradiation of 1000 rad, 2000 rad and 3000 rad. The Ga-67 uptake in animal tumor decreased by irradiation of 1000 rad, 2000 rad and 3000 rad. The histological change of tumor tissue revealed a marked degeneration and necrosis by irradiation. The growth of cultured tumor cell (mouse leukemia cell: L5178Y) was inhibited by Adriamycin of antineoplastic agent. However, Ga-67 uptake in cultured tumor cells increased by addition of Adriamycin. (2) the relationships between the Ga-67 uptake and ATP metabolism in cultured tumor cell was investigated. The effects of NaF an Ouabain which is inhibitor of ATP metabolism, on the Ga-67 uptake in cultured cell were examined. The Ga-67 uptake in NaF and Ouabain treated cells decreased as compared with that of control cell. (3) The relationships between the Ga-67 uptake and cytoskeletal filaments of tumor cell was investigated. After addition of Cytochalasin B, Ga-67 uptake in tumor cell was significantly decreased. It found that cytoskeletal filaments of cell play an important role on Ga-67 uptake in tumor cell.2. Clinical trialsThe accumulation of Ga-67 in a tumor has been found to diminish after effective irradiation of chemotherapy. To explore this problem further, we investigated the change of Ga-67 scintigram following irradiation and chemotherapy for cases with malignant tumor of the neck region.Ga-67 scintigrams of all cases of malignant tumor revealed positive images before treatment. However, after effective therapy the Ga-67 scintigrams became negative. This observation suggested that Ga-67 scintigrams can provide useful information regarding therapeutic effectiveness.