Immobilization of enzymes and antibodies on magnetic particles isolated from magnetotactic bacteria

我们已经开发了一种新颖的用于磁性细菌孤立的磁性粒子的方法。Magnetotactic bacteria were incubated at 25 C for a fwe weeks in the sludge enriched with 0.005%sodium succinate and 0.005%sodium nitrate， and collected by use of a magnetic harvesting apparatus。磁性细菌主要是Cocci和rod型。这些细菌大约含有10到20个磁性粒子。Biogenic magnetic particles were separated form magnetotactic bacteria by digestion with NaOH。磁性粒子的尺寸为500-1000<ang>，是磁铁（Fe_3O_4）的一个微小的体积，由能量分散的X射线分析仪和高分辨率传输电子显微镜分析。This particles were coverd with organic thin films。Immobilization of enzymes and antibodies on biogenic magnetic particles were carried out by treating magnetic particles with<gamma>-（aminopropyl）triethoxysilane and glutaraldehyde， and incubated with antibodies and enzymes at 4 C for 12 hr。Large（大） ... More amounts of enzymes and antibodies were immobilized on biogenic magnetic particles because of its uniform sharp and small size。Enzymes immobilized on magnetic particles show high activity.We measured glucose concentration by using glucose oxidase immobilized on magnetic particles and optic fiber sensor.葡萄糖浓缩可在0.1-4.0毫克/毫升的范围内进行线性测量。Enzyme反应是由磁性刺激增强的。E.大肠杆菌的选择性分离也是通过用于抗E.大肠杆菌抗体不流动于磁性颗粒上的。在磁性地缠绕和分离后，E.大肠杆菌细胞集中分解。在这些细胞中，L.酸性芽孢杆菌和S.细胞在磁性分离后没有分解的地方。因此，特定细胞可能是分开的选择。Furthermore，一小部分抗原的体积可以通过微观观察快速检测到抗原--抗体反应形成聚合物通过使用抗抗体在磁性粒子上的非移动性来形成。生物质磁性粒子被承诺用于未来在临床领域的应用。Less（低）

We have developed the novel use of magnetic particles isolated from magnetotactic bacteria. Magnetotactic bacteria were incubated at 25゜C for a fwe weeks in the sludge enriched with 0.005% sodium succinate and 0.005% sodium nitrate, and collected by use of a magnetic harvesting apparatus. Magnetotactic bacteria were mainly cocci and rod type. These bacteria contained about 10 to 20 magnetic particles. Biogenic magnetic particles were separated form magnetotactic bacteria by digestion with NaOH. The magnetic particles were 500-1000<ang> in size and aminly consist of magnetite (Fe_3O_4), which analysed by an energy dispersive X-ray analyzer and a highresolution transmission electron microscope. This particles were coverd with organic thin films.Immobilization of enzymes and antibodies on biogenic magnetic particles were carried out by treating magnetic particles with <gamma>-(aminopropyl)triethoxysilane and glutaraldehyde, and incubated with antibodies and enzymes at 4゜C for 12hr. Large … More amounts of enzymes and antibodies were immobilized on biogenic magnetic particles because of its uniform sharp and small size. Enzymes immobilized on magnetic particles show high activity.We measured glucose concentration by using glucose oxidase immobilized on magnetic particles and optic fiber sensor. Glucose concentration could be measured linearly in the range of 0.1-4.0 mg/ml. Enzyme reaction was enhanced by magnetic stirring. Selective separation of E.coli was also done by use of anti-E.coli antibody immobilized on magnetic particles. After magnetically stirring and separation, E.coli cell concentration decreased. Whereas the cell concentrations of L.acidphillus and S.cervisiae didn't decreased after magnetic separation. Therefore, particular cell could be separated selectivity. Furthermore, the small amount of antigen could be detected rapidly by microspopic observation of antigen-antibody reaction forming agglutination by use of the antibodies immobilized on magnetic particles. Biogenic magnetic particles are promising for the future application in clinical field. Less