Adjuvant therapy for lung cancer. - a missile therapy using monoclonal antibody

为了达到对手术肺癌的完美辅助治疗，我们提出了所谓的“导弹治疗”，并做了以下工作。1)制备高效肺癌相关单克隆抗体（MOAB）。2)研究与人肺癌组织及其他肿瘤病例的相互作用，同时通过免疫组化染色将MOAB纳入正常器官，从而寻求其定位。3)制备人肺癌移植裸鼠，将I^<125>偶合的MOAB注入动物体内，用放射自显影检查其与靶细胞的结合或在其他器官的定位。4)选择有效的MOAB并与抗癌药物偶联，观察其疗效。结果发现，我们建立的MOAB可用于病理诊断，但由于某些原因，不适合用于导弹治疗。随后，我们用MOAB反应Le^x-i抗原进行了类似的反应，这是2型葡萄糖偶联抗原，结果发现它对肺腺癌表现出特别强的反应性，与正常肺组织的交叉反应仅在支气管腺中表现出来。因此，我们将该偶联I^<125>的MOAB注入已制备的裸鼠体内，并对其进行切片，发现其有效性，现在制成了含有MOAB的ADR脂质体。

The so called missile therapy in order to achivev the perfect adjuvant therapy against operated lung cancer were intended, and the following works ere made.1) Preparation os highly effective monoclonal antibody (MOAB) associated with lung cancer. 2) Investigation of the mutual reactions with the cases of human lung cancer tissues and other neoplasms, and simultaneously of the incorporation of MOAB into normal organs by immunohistochemical staining, thus pursuing its localization. 3) Preparation of nude mice on which human lung cancer was transplanted, and MOAB conjugated with I^<125> was infused into the animals, anmd the incorporation into the trrget or localization in other organs were examined with autoradiography. 4) Decision of effective MOAB and conjugation with anti-cancer drugs were made to investigate the effect.It was found as a result that the MOAB established by us was useful for pathological adiagnosis, but it was found inappropriate for missile therapy for some reasons. Subsequently, we experimented a similar reaction with a MOAB reacting Le^x-i antigen, which is type 2 glucose-cojugated antigen and it was found as a result that it showed a specifically strong reactivity to adenocarcinoma of the lung, and its crossreaction with the normal lung tisssue was shown only in the bronchial gland. Therefore, we infused this MOAB conjugated with I^<125> into already prepared nude mice, and their sections was made its usefulness was found out, and now the ADR liposome with MOAB is made.