Investigation of immunologic heterogeneity of primary head and neck cancer and its cervical metastases.

通过测定12例头颈部肿瘤患者对细胞毒性淋巴细胞（CL）的敏感性，观察其原发肿瘤与颈部淋巴结转移灶之间的抗原差异。采用自体混合淋巴细胞肿瘤细胞培养（MLTC）和重组白细胞介素-2（il -2）培养诱导细胞毒性。本研究使用的效应细胞包括OKT3+8+和OKT3+4+亚群。它们的细胞毒性与淋巴因子激活的杀伤细胞（LAK细胞）活性不同。12例患者中有7例（58.3%）观察到自体原发肿瘤细胞（CLp）刺激CLs的细胞毒性。相比之下，12例患者中有4例（33.3%）观察到转移性肿瘤细胞（CLm）刺激的CLs细胞毒性。在成功诱导CLp和CLm的情况下，进行交叉反应性试验和冷靶抑制试验。这些结果表明，免疫原性的降低发生在转移部位，对自体CL的敏感性在原发和转移肿瘤细胞之间是不同的。

Antigenic differences between primary tumors and their cervical lymph node metastases of 12 patients with head and neck cancers were examined by measuring their sensitivity to cytotoxic lymphocytes(CL). Cytotoxicity was induced by autologous mixed lymphocyte tumor cell culture (MLTC), and further cultivation with recombinant interleukin-2(rIL-2). The effector cells which were used in this study consisted of OKT3+8+ and OKT3+4+ subpopultions. Their cytotoxic nature was different from lymphokine activated killer cell (LAK cell) activity. Cytotoxicity of CLs stimulated by autologous primary tumor cells(CLp) was observed in 7 out of 12 patients(58.3%). In contrast, cytotoxicity of CLs stimulated by metastatic tumor cells (CLm) was observed in 4 out of 12 patients (33.3%). In the cases in which both CLp and CLm were successfully induced, cross-reactivity tests and cold target inhibition tests were performed. These results suggested that a reduction in immunogeneicity had occurred at the metastatic site, and sensitivity against autologous CL was different between primary and metastatic tumor cells.