Polymorphonuclear leukocytes as an inflammatory hormone-producing argan.

多形核白细胞(PMN)被认为是外来物质的清道夫细胞。最近，我们已经意识到PMN除了具有众所周知的清道夫作用外，还可能具有重要的炎性激素产生细胞的作用，因为我们已经注意到PMN在兔腹膜炎症的早期阶段产生了免疫增强因子。这种免疫增强因子被认为是中性粒细胞渗入炎症部位后新合成的。这一假设的基础是：(A)血液中的PMN不存在这种因素。(2)炎症性PMN迅速改变，在炎症部位具有该因子。(C)联合内毒素和摇床培养可在体外触发血PMN产生该因子。这种体外合成被几种蛋白质合成抑制剂完全抑制。(D)~(14)C标记的氨基酸被引入到现在合成的im…中。更多的MUN增强因子。我们分离了炎性免疫增强因子并确定了其部分结构。此外，我们还成功地筛选出了编码免疫增强因子的cDNA克隆，并测定了其结构。由于巨噬细胞通常被认为是产生IL-1的主要细胞，但我们最终证明了：(A)从早期炎性腹膜渗出细胞中提取的高度纯化的PMN含有IL-1。(B)纯化的PMN表达IL-1。(C)组织化学证实炎症部位约99%的含有IL-1的细胞为PMN。(D)在整个炎症过程中，只有少数含有IL-1的细胞是巨噬细胞。IL-1目前被认为是一种重要的炎症激素，我们认为PMN在炎症部位也可能产生除IL-1以外的重要物质。我们研究了这种可能性，发现PMN在长时间的炎症中保持了蛋白质合成能力，直到48小时。炎性PMN到达炎症部位后至少产生8种分泌蛋白。这些新合成的物质的生物生物学功能有待进一步研究。较少

Polymorphonuclear leukocytes (PMN) have been believed to serve as scavenger cells for foreign substances. Just recently, we have realized that PMN may have an important role as an inflammatory hormone-producing cells in addition to the well known role as scavengers, because we have noted that PMN were producing an immune potentiation factor during the early stage of inflammation in peritoneal cavity of rabbits. This immune potentiation factor was considered to be newly synthesized by PMN after their infiltration into the inflammatory site. The basis of the assumption was (a) Blood PMN do not have such factor. (b) Inflammatory PMN quickly changed to have the factor at the inflammatory site. (c) Blood PMN could be triggered to produce the factor in vitro by stimulation of a combination with endotoxin and shaking culture. This in vitro production was completely inhibited by several inhibitors of protein synthesis. (d) 14C-labelled amino acids were incorporated into the nowly syntheized im … More mune potentiation factor.We have isolated the inflammatory immune potentiation factor and determined its partial structure. Furthermore, we have succeeded to choose a cDNA clone coding fot the immune potentiation factor and determined its structure. The structure closely resembled that of human and murine IL 1 then, we concluded that this inflammatory factor is IL 1 of rabbit.Then, we were faced the problem of consideration whether this IL 1 is really produced by PMN, because macrophages were generally believed to be the major producer of IL 1 but we finally proved the production of IL 1 by PMN as follows: (a) A highly purified PMN from the early inflammatory peritoneal exudate cells contained IL 1. (b) Also, the purified PMN express the mRNA for IL 1 .(c) It was histochemically proved that about 99% of IL 1 -containing cells at inflammatory site were PMN. (d) Only a few percentage of IL 1 -containing cells were macrophages during the entire course of the inflammation. The IL 1 is now considered to be one of important inflammatory hormone and we considered that PMN also may produce important substances other than IL 1 at the inflammatory site. We investigated this possibility and found that PMN kept their protein synthesis capability during a long period of inflammation until 48 hrs. The inflammatory PMN produced at least 8 kinds of secreting proteins after arrival into the invlammatory site. The biolobical functions of these newly syntheseized substances are the matter of further investigation. Less