Specific restriction fragment length polymorphism on HLA region susceptibility to psoriasis vulgaris

本研究旨在探讨寻常型银屑病易感基因是否存在于人类白细胞抗原，尤其是人类白细胞抗原C区。人类白细胞抗原C区限制性片段长度多态性与寻常型银屑病易感性基因组DNA的Southern杂交结果显示，无论是Cw6还是Cw7，寻常型银屑病患者均未发现人类白细胞抗原C基因的DNA多态性。然而，在一些患有HLACw6或Cw7的健康对照组中，缺失了4.5kb的BamHI片段和3.1kb的PstI片段。这些特定的片段可能有助于个人患寻常型银屑病，也可能是疾病发展所必需的。研究了人类白细胞抗原Cw6和Cw7的重要氨基酸序列，并与已发表的人类白细胞抗原Cw1、Cw2、Cw3、Cw6、Cw7、Cw11和本实验室检测到的Cx52的氨基酸序列进行了比较。第9位和第90位天冬氨酸是人类白细胞抗原Cw6和Cw7的重要氨基酸序列。尤其是人类白细胞抗原C抗原的第9位氨基酸序列是免疫学上的重要位置之一。因此，在寻常型银屑病中，这种氨基酸可能是这种疾病发生和/或发展所必需的。人类白细胞抗原在寻常型银屑病中的作用根据我们的研究结果，讨论了一些关于人类白细胞抗原在寻常型银屑病中作用的假说。

This study was carried out to investigate whether or not the gene controlling the susceptibility to psoriasis vulgaris existed on the HLA, especially the HLA-C region.1. Restriction fragment length polymorphism on HLA-C region and susceptibility to psoriasis vulgarisSouthern hybridization of genomic DNA demonstrated that DNA polymorphisms of the HLA-C antigen gene could not be found in any psoriasis vulgaris patient whether HLA-Cw6 or Cw7. However, a 4.5 kb BamHI fragment and a 3.lkb PstI fragment were lacking in some healthy controls who had either HLA-Cw6 or Cw7. These specific fragments may help predispose individuals to psoriasis vulgaris, or may be essential for the development of the disease.2. Significant amino acid sequences of HLA-Cw6 and Cw7 antigensSignificant amino acid sequences of HLA-Cw6 and Cw7 antigens were investigated to compare with amino acid sequences of HLA-C antigens each other;namely HLA-Cwl, Cw2, Cw3, Cw6, Cw7, Cwll, which were already published, and Cx52, which was detected in our laboratory. Aspartic acids of position 9 and 90 were recognized as significant amino acid sequences of HLA-Cw6 and Cw7 antigens. Especially,the positon 9 amino acid sequences of HLA-C antigen is immunologically one of important positions. So that, in psoriasis vulgaris, this amino acid may be essential as to the onset and/or development of this disease.3. Roles of HLA in psoriasis vulgarisFrom our results, some hypotheses were discussed as to roles of HLA antigens in psoriasis vulgaris.