Electron Microscopic Studies on Osteoclastic Bone Resorption

本研究旨在探讨几种条件下破骨细胞性骨吸收的过程，阐明破骨细胞的结构和功能以及骨吸收的机制。结果如下：1.破骨细胞性骨吸收的比较研究：采用组织学、扫描电子显微镜和透射电子显微镜对大鼠骨吸收区域进行观察。扫描电子显微镜观察到的结果与组织学和透射电子显微镜的结果一致。这表明比较形态研究是必要和有用的程序之一。吲哚美辛对破骨细胞性骨吸收的影响：为了探讨吲哚美辛对实验性牙周组织炎性损伤大鼠破骨细胞性骨吸收的影响，采用组织学和超声波法…观察了吲哚美辛对牙周炎性损伤大鼠牙间骨吸收的影响。更具乡土气息。IM组大鼠破骨细胞性骨吸收减少。骨吸收活性的降低似乎是IM抑制前列腺素(PGs)合成的结果。提示PGs在牙周组织炎症的破骨细胞性骨吸收中起重要作用。破骨细胞性骨吸收在拔牙创面愈合过程中的作用：为探讨骨吸收在拔牙创面愈合过程中的作用，对IM组大鼠的骨吸收过程进行了组织学和超微结构观察。IM组大鼠因破骨细胞性骨吸收抑制和新骨形成减少而导致骨不完全改建，从而延缓愈合过程。这些发现表明IM抑制PG的合成，从而降低骨吸收和重塑的活性。因此，认为PGs作为一种化学介质也参与了拔牙创面的愈合过程，破骨细胞与成骨细胞在这一过程中起着重要的作用。较少

The present studies were intended to investigate the process of the osteoclastic bone resorption under several conditions and to clarify the structures and functions of osteoclasts and the mechanism of bone resorption. The results were summarized as follows:1. Comparative studies on osteoclastic bone resorption: The regions of bone resorption in rats were examined by histologic, scanning electron microscopic(SEM) and transmission electron microscopic(TEM) methods. The findings observed by SEM were consistent with the histological findings and those of TEM. It indicated that the comparative morphological investigation is one of the necessary and useful procedures.2. Effect of Indomethacin(IM) on osteoclastic bone resorptioin: In order to investigate the effect of IM on osteoclastic bone resorption in experimentally induced inflammatory lesion of periodontal tissue in rats, the resorption at the interdental bone induced by insertion of elastic band was examined histologically and ultrast … More ructurally. In the IM administrated rats, the reduction of the osteoclastic bone resorption was observed. It seems that the reduction of the bone resorbing activity is a consequence of the inhibition of prostaglandins (PGs) synthesis caused by IM. These results suggest that PGs play an important role on the osteoclastic bone resorption in inflammation of periodontal tissues.3. Osteoclastic bone resorption in the healing process of the extraction wound: In order to investigate the role of the bone resorption in the healing process of the extraction wound, the process in IM administrated rats was examined histologically and ultrastructurally. In the IM administrated rats, the retardation of healing process related to incomplete bone remodeling, which was caused by the inhibition of osteoclastic bone resorption and the reduction of new bone formation, was observed. These findings suggest that IM inhibits pgs synthesis and consequently reduces the activity of bone resorption and remodeling. Thus, it is considered that PGs as one of the chemical mediators perticipate also in the healing process of extraction wound and that osteoclasts, associated with osteoblasts, play an important role in this process. Less