Relationship between Caries-detector Stainability and Ultrastructure of Carious Dentin

本研究旨在改进龋齿检测仪，使其在龋齿外牙本质的渗透性和染色性较差，因此需要较长的治疗时间。用6种不同溶剂配制的酸红溶液对人工脱钙的牛牙本质和提取的人龋齿牙本质进行了体外透性测定。另外，通过人工脱钙牙本质渗透性试验，评价了染料浓度和涂抹时间对脱钙牙本质渗透性的影响。因此，选择DMSO作为实验龋检测器溶液的合适溶剂。在实验检测器中加入10%柠檬酸，去除牙本质切割表面的涂抹层。设计了10%柠檬酸-1%酸红-10% DMSO溶液作为新型龋探测器。在体外和体内对新型检测器的效率进行了比较，发现了以下事实；DMSO溶液对人工脱钙牙本质的渗透性最好。在丙二醇、乙二醇和DMSO溶液中，DMSO溶液对外龋牙本质的渗透效果最好，表明DMSO溶液对人工脱钙牙本质的渗透性较好。应用时间越长，1%酸性红丙二醇溶液对人工脱钙牙本质的渗透性越好。高浓度染料还使上述溶剂制成的酸性红溶液对人工脱钙牙本质和龋齿牙本质具有更大的渗透性。然而，经常使用5%酸性红溶液，容易使侧腔壁完整的牙本质表面染色。在使用检测液之前，用薄柠檬酸去除涂抹层，龋齿检测频率显著降低。

This study was projected to improve the caries detector which had less stainability and permeability into the outer carious dentin and so required longer chair time for caries treatment. Permeability of acid red solutions made of six types of solvents were determined in vitro for an artificially decalcified bovine dentin and exracted human carious dentin. In addition, the effects of dye-concentration and applying time of these solutions were evaluated through the permeability test in the artificially decalcified dentin. As a result, DMSO was selected as a suitable solvent for an experimental caries detector solution. 10% citric acid was added to the experimental detector for removing the smeared layer on cut dentin surfaces. The 10% citric acid-1% acid red-10% DMSO solution was thus designed as a new caries detector. Efficiency of the new detector was also examined in vitro and in vivo compared with the conventional, finding the following facts;1. DMSO solution was the most permeable into the artificially decalcified dentin among six types of solutions.2. DMSO solution showed the most permeable effect in the outer carious dentin among propylene glycol, ethylene glycol and DMSO solution, which indicated relatively good permeability into the artificially decalcified dentin.3. The longer applying time exhibited great permeability of 1% acid red propylene glycol solution to the artificially decalcified dentin. High concentration of the dye also brought in greater permeability of the acid red solutions made of the above-mentioned solvents into both the artificially decalcified and the carious dentin. The frequent use of 5% acid red solution, however, was liable to stain the intact dentin surfaces of the side cavity walls.4. When the smeared layer was removed with thin citric acid prior to use of the detector soultion, frequency of caries detection remarkably decreased.