微生物細胞捕捉機能を有する高分子材料の合成及びバイオリアクタ-への応用

首席研究员发现了一种新的功能性聚合物，不溶性吡啶型树脂，可以捕获活的微生物。本课题旨在开发一种基于树脂的微生物细胞固定化新方法，并将其应用于生物反应器。1988学年，采用捕集法将具有天冬氨酸酶活性的大肠杆菌全细胞固定在树脂表面。当缓冲溶液中的细菌细胞悬浮液通过含有树脂珠的玻璃柱时，细胞被捕获在表面并形成固定化细胞体系，用于从富马酸铵制备l -天冬氨酸。固定床塔式反应器的连续操作。在pH为7.4-7.7,30‰的条件下，当进水底物浓度为0.1 M，流速为0.41-0.83床体积/小时时，细菌细胞定量产l -天冬氨酸。1989学年，将具有3-酮类固醇- delta ^1-脱氢酶活性的单纯节杆菌细胞捕获固定在涂有吡啶型聚合物的无纺布表面。捕获的细胞在甲醇溶剂中催化皮质醇转化为强的松龙。在20℃、pH 8.2条件下，入液皮质醇浓度为1.0 mmol/1，流速为0.57床体积/ h，固定床柱反应器连续操作，强的松龙的产率约为90%。

The head investigator has found a new functional polymer, insoluble pyridiniumtype resin, which captures microorganisms alive. The purpose of this research project was to develop a new method of immobilization of microbial cells based on the resin, and to apply for bioreactor. In academic year of 1988, whole cells of Escherichia coli with aspartase activity were immobilized by capture on the surface of the resin. When a suspension of the bacterial cells in buffer solution was passed through a glass column containing beads of the resin, the cells were captured on the surface and formed an immobilized cell system, which was used for the preparation of L-aspartic acid from-ammonium fumarate. Continuous operation of a fixed-bed column reactor containing the unmobilized . bacterial cells produced L-aspartic acid in a quantitative yield when the influent substrate concentration was 0.1 M and the flow rate was 0.41-0.83 bed volumes per hour at pH 7.4-7.7 at 30ﾟC. In academic year of 1989, cells of Arthrobacter simplex having 3-ketosteroid-DELTA^1-dehydrogenase activity were immobilized by capture on the surface of unwoven cloth coated by pyridinium-type polymer. The captured cells catalyzed the transformation of cortisol to prednisolone in methanolic solvents. Continuous operation of a fixed-bed column reactor gave prednisolone in about 90% yield when the influent cortisol concentration was 1.0 mmol/1 and the flow rate was 0.57 bed volumes per hour at 20ﾟC and at pH 8.2.