On the Studies of the Sterilized Egg Appearance and Their Metabolism in the Silkworm

绝育卵的出现给蚕种生产带来了很大的经济损失。为了解决这些问题，已经进行了许多研究，并提出了许多观点。但仍有更多问题有待解决。因此，作者计划进行后续实验，以明确绝育卵出现的机制。1)将绝育蛋品系与三蜕皮品系杂交，进行多种遗传组合。在下一代中，对灭菌蛋产品批次进行了研究。因此，推测绝育基因定位于6条染色体和三蜕皮基因位点。2)为探讨鸡蛋成分与无菌蛋外观的关系，研究了氨基酸、蛋白质和脂肪酸组成。无菌卵与正常卵在上述物质上存在一定差异。除菌蛋的异亮氨酸、天冬氨酸含量明显高于正常蛋，谷氨酸、丙氨酸、蛋氨酸、赖氨酸含量明显低于正常蛋。否则，在脂肪酸组成中，前者的亚麻酸比后者少得多。3)用细胞分选仪研究了注射幼激素后性腺及其精细胞的发育情况。结果表明，幼激素抑制了性腺和精子的发育，控制了细胞分裂的G_1期。综上所述，我认为绝育卵子的外观是由许多遗传和非遗传因素控制的。

Appearance of sterilized eggs give much economical damage for seed production in the silkworm. Many studies have been carried out to resolve these problems and many points were declared. But much more issuestill remain to be done. So that author planed an following experiments to clear the mechanism for the appearance of sterilized eggs.1) Sterilized eggs strain was crossed with tri-molting strain and made a many genetical combinations. In following generation, sterilized eggs product batches were investigated. As a result, it is speculated that sterilized gene localize on the six chromosome as well as tri-molting gene loci.2) In order to investigate the relation to an appearance of sterilized eggs and egg components, amino acid, protein and fatty acid composition were investigated. There are some differences between sterilized and normal eggs in above substances. Sterilized eggs have much more than normal ones in isoleucine and aspartic acid and less in glutamic acid, alanine, methionine, lysine. Otherwise, in fatty acid composition, the former one has much less linolenic acid than later one.3) Developments of gonad and its spermatids injected juvenile hormone were investigated by cell sorter. It was declared that juvenile hormone inhibited a development of the gonad and spermatids and control the G_1 stage in cell division.From above results, I consider that appearance of sterilized eggs are controlled by the many genetical and non-genetical factors.